The lesion mimic mutant lmm4 was identified from an EMSinduced Nipponbare mutant library. Brown lesions were first observed on the tip of lmm4 leaves at the initial tillering stage, and spreaded gradually downward to the whole leaf blade and even the leaf sheath. Compared with the wild type, the chlorophyll and the net photosynthetic rate of lmm4 were significantly reduced. The plant height, panicle length, seed setting rate, grain weight were also decreased significantly. The shading assay showed that the lesions on lmm4 leaves were induced by natural light. The histological staining assay indicated that the lesions on lmm4 leaves were caused by programmed cell death and hydrogen peroxide accumulated in lmm4 leaves. The lmm4 displayed higher resistance to blast race C7 and G1 when compared with the wild type. In addition, pathogenesisrelated gene POC1, PAL,PBZ1, PR1 were found to be upregulated in lmm4. Genetic analysis suggested that the phenotype of lmm4 was controlled by a single recessive nuclear locus. Based a mapbased cloning strategy, the lmm4 was narrowed down to a 235 kb region between Ind4 and Ind6 near the centromere of chromosome 5.
QIU Jiehua1, MA Ning1, 2, JIANG Hanwei1, 2, SHENG Zhonghua1, SHAO Gaoneng1, TANG Shaoqing1, WEI Xiangjin1, *, HU Peisong1, *
. Identification and Gene Mapping of a Lesion Mimic Mutant lmm4 in Rice[J]. Chinese Journal OF Rice Science, 2014
, 28(4)
: 367
-376
.
DOI: 10.3969/j.issn.1001-7216.2014.04.005
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