By 60Co rray radiation, a small grain and dense panicle mutant sep1 was obtained from the indica variety Shuangkezao. Compared to the wild type Shuangkezao, it was a mutant with smaller grain, shorter spikestalk but more grain number per panicle. The histological slice observation of the main stem showed that the mutant had more vascular bundle number in one cycle and more cell number per unit area than the wild type. Genetic and allelism analysis results showed that the small grain and dense panicle mutant was controlled by a recessive gene, and it was different to the dense panicle genes which had been already cloned. Then by constructing the genetic segregated population and using the InDel markers, the small grain and dense panicle gene was mapped on the centromeric region of chromosome 5 between marker XP2628 and XP2631. In addition, the breeding application of the mutant was carried out on CMS lines and the stabile lines of high generations had finally been obtained.
WANG Yuexing1,＃, WU Kun2,＃, FANG Yunxia1, CHEN Hongqi1, NI Shen1, FU Xiangdong2, ZHU Xudong1,*
. Genetic Analysis, Gene Mapping and Breeding Application of Small Grain and Dense Panicle Mutant sep1 in Rice[J]. Chinese Journal OF Rice Science, 2014
, 28(3)
: 229
-234
.
DOI: 10.3969/j.issn.1001-7216.2014.03. 002
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