This study was conducted to validate a quantitative trait locus (QTL) for 1000grain weight (TGW) which was previously mapped in a 3.7 Mb region on the long arm of rice chromosome 1, and to locate this QTL more precisely. A plant carrying a heterozygous segment extending from RM11448 to RM11615 was selected from the Zhenshan 973/Milyang 46 BC2F5 population. The resulting BC2F6 and BC2F7 populations were assayed with SSR markers. Three BC2F7 plants were identified, carrying heterozygous segments covering the intervals RM11448-RM11522, RM11448-RM11549 and RM1232-RM11615, respectively. Nonrecombinant homozygotes were identified from the BC2F8 populations, from which three sets of near isogenic lines (NILs) in the BC2F8∶9 generation were produced, consisting of two parental genotypes in the intervals RM11448-RM11522, RM11448-RM11549 and RM1232-RM11615, respectively. Field trials were conducted to measure heading date (HD), TGW, and grain shape traits including grain length and width. Twoway analysis of variance was performed to test the phenotypic differences between two genotypic groups in each NIL set using SAS program. No significant variations were observed on TGW and grain shape in the NIL which was heterogeneous in the interval RM11448-RM11522, whereas significant genotypic effects were detected in the remaining two NILs in which the enhancing alleles were all derived from Zhenshan 97. For HD, no significant effects were detected in the three NILs. Based on comparison among genomic locations of the three heterogeneous regions, qTGW1.1 was delimited to a 688.8 kb region flanked by RM11522 and RM11554.
ZHANG Hongwei, ZHU Yujun, CHEN Yuyu, CHEN Junyu, HUANG Derun, ZHUANG Jieyun*
. Validation of qTGW1.1, a Quantitative Trait Locus for 1000grain Weight in Rice (Oryza sativa L.)[J]. Chinese Journal OF Rice Science, 2014
, 28(2)
: 125
-131
.
DOI: 10.3969/j.issn.1001-7216.2014.02.003
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