Occurence of Heterodera elachista in Guangxi Region and Its Intraspecies Heterogeneity&nbsp; in rDNAITS Region

ZHUO  Kan1, SONG Handa1, WANG Honghong1, TAO Ye1, ZHANG Hongling1, LU Xiuhong2, HUANG Jinling2, LIU Zhiming2, LIAO Jinling1,*

doi:10.3969/j.issn.1001-7216.2014.01.011

Chinese Journal OF Rice Science >

2014 , Vol. 28 >Issue 1: 78 - 84

DOI: https://doi.org/10.3969/j.issn.1001-7216.2014.01.011

Research Papers

Occurence of Heterodera elachista in Guangxi Region and Its Intraspecies Heterogeneity in rDNAITS Region

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¹Laboratory of Plant Nematology, College of Natural Resource and Environment, South China Agricultural University, Guangzhou 510642, China; ²Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China;

Received date: 2013-07-30

Revised date: 2013-10-01

Online published: 2014-01-10

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Abstract

In 2011, a cyst nematode was isolated from rice roots and soils in Longsheng Terrace of Guangxi Region, China. Morphological characteristics of the cyst nematode were consistent with previous descriptions of Heterodera elachista. The rDNAITS sequences of the cyst nematode and H. elachista in GenBank shared 97.7%-99.3% identities. The phylogenetic tree based on rDNAITS showed that the cyst nematode was in the same monophyletic clade as H. elachista with 100% support and short branch differences. The results indicated the cyst nematode was Heterodera elachista. The restriction enzyme cutting sites of ten rDNAITS sequences of H. elachista amplified and the one reported by Tanha Maafi (GenBank accession number: AF498391) were analyzed. The restriction enzyme cutting sites of AluⅠ, AvaⅠ, MvaⅠ and RsaⅠ in these 11 sequences were identical, however there were 2 different patterns after Bsh1236Ⅰ and BsuRⅠ digestion, respectively, and 3 different patterns after CfoⅠ digestion. The results show intraspecies heterogeneity in rDNAITS region exists in H. elachista.

Key words： Heterodera elachista; rice; identification; rDNAITS; heterogeneity

Cite this article

ZHUO Kan1, SONG Handa1, WANG Honghong1, TAO Ye1, ZHANG Hongling1, LU Xiuhong2, HUANG Jinling2, LIU Zhiming2, LIAO Jinling1,* . Occurence of Heterodera elachista in Guangxi Region and Its Intraspecies Heterogeneity in rDNAITS Region[J]. Chinese Journal OF Rice Science, 2014 , 28(1) : 78 -84 . DOI: 10.3969/j.issn.1001-7216.2014.01.011

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