Effects of exogenous silicon on spikelet fertility of hybrid rice were investigated at natural high and normal temperatures during anthesis under field conditions with two hybrids, hightemperature sensitive Jinyou 63 and hightemperature tolerant Shanyou 63 as material. The exogenous silicon via fertilizer application, in the form of sodium silicon (Na2SiO3·9H2O), was consecutively spayed for three times at jointing stage at four concentrations, 0 mmol/L (TCK), 1.25 mmol/L(T1.25 ), 2.50 mmol/L (T2.50), and 3.75 mmol/L(T3.75 ). Varietal responses in terms of pollination properties, anther dehiscence and spikelet pollination rate were examined to establish a feasible screening scheme of the most appropriate sodium silicate application level in rice management practices. Results showed that, sodium silicate applications at the concentration of 1.25 mmol/L, 2.50 mmol/L or 3.75 mmol/L under high temperature in field environments effectively increased the germinated pollen number per stigma by 16.9% and 39.6% for the heatsensitive Jinyou 63 and heattolerant Shanyou 63, respectively. The number of pollen grains per stigma increased by 34% and 305%, respectively. The pollen germination rate rose by 11.3 and 7.0 percentage points, respectively; the percentage of florets with more than 10 germinated pollen grains was increased by 9.3 and 27.6 percentage points, respectively; while the percentage of florets with fewer than 20 pollen grains was reduced by 18.8 and 8.1 percentage points, respectively. Application of the silicon fertilizer also increased anther basal dehiscence width by relative values of 19.0% and 10.3%, respectively, while it improved spikelet pollination rate by absolute values of 10.6 and 4.7 percentage points in the sensitive and tolerant hybrids, respectively. These results showed that the effect of silicon in alleviating fertility reduction may be mainly attributed to the pollen quality improvement, but not the pollen quantity or the anther dehiscence. Exogenous silicon was more effective for hightemperature sensitive combination than that for the tolerant and the most effective dosage was at the concentration of 2.50 mmol/L.
WU Chenyang1, YAO Yimin1, SHAO Ping1, WANG Yi1, WANG Zhiwei1, TIAN Xiaohai1,2,*
. Exogenous Silicon Alleviates Spikelet Fertility Reduction of Hybrid Rice Induced by High Temperature under Field Conditions[J]. Chinese Journal OF Rice Science, 2014
, 28(1)
: 71
-77
.
DOI: 10.3969/j.issn.1001-7216.2014.01.010
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