A rice mutant characterized by yellowgreen leaves (named as ygl10) was obtained from an indica cultivar 9311 via radiation of 60Coγ ray. Morphological analysis showed that, the ygl10 mutant exhibited shorter plant height and panicle length, decreased seed setting rate compared to its wild type (9311). Moreover, the chlorophyll content in the ygl10 mutant leaves dramatically decreased, especially for chlorophyll b content, being only about 2% of that in its wild type. In addition, the SEM results showed that the numbers of grana lamella, and thylakoids in the mutant were less than those in the wild type. Genetic analysis and gene mapping showed that the yellow green leaf mutant was controlled by a single recessive gene, which was located in a 380 kb region on chromosome 10. We further analyzed the putative candidate open reading frames in the target region through sequencing, and the results showed that there was a 5nucleotide deletion in the ninth exon of CAO1 gene (encoding chlorophyll a oxygenase involved in the biosynthesis of chlorophyll b), resulting in the frame shift to generate a new premature stop codon. Therefore, it is deduced that CAO1 is the candidate of ygl10.
YANG Hailian#, LIU Min#, GUO Min, LI Rongde, ZHANG Honggen, YAN Changjie*
. Genetic Analysis and Position Cloning of a yellowgreen leaf 10 (ygl10) Gene, Responsible for Leaf Color in Rice[J]. Chinese Journal OF Rice Science, 2014
, 28(1)
: 41
-48
.
DOI: 10.3969/j.issn.1001-7216.2014.01.006
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