Rice false smut, caused by Ustilaginoidea virens, is an important disease in rice panicle. Degenerate PCR primers were designed according to the conserved amino acid sequence of several filamentous fungus mitogen activated protein kinase(MAPK) homologous to yeast Hog1 MAPK, and partial DNA fragment encoding a MAPK was amplified from U. virens through PCR. Then, a whole DNA sequence encoding the MAPK, designated as UvHog1, was obtained from U. virens by extending upstream and downstream sequence of the amplified fragment using RACE method. And its amino acid sequence shared 95%, 93%, 88% and 81% identity to MgOsm1 （AAF09475.1） from Magnaporthe grisea, BbHog1 （AAS77871.1） from Beauveria bassiana, AfOsm1（XP752664.1）from Aspergillus fumigatus and CrHog1（AAM26267.1）from Cryptococcus neoformans var. neoformans, respectively. Phylogenetic clustering suggested that UvHog1 is homologous to yeast Hog1 MAPK. The results of the decreased transcript levels of UvHog1 by salinity suggest that UvHog1 may be involved in the specifically responses to salt stresses.
RAO Yuchun1,2,#, DING Zhengzhong1,3,#, CHEN Xifeng1, ZENG Dali2, MA Bojun1, GU Zhimin1,*
. Cloning and Expression Analysis of UvHog1 Gene in Ustilaginoidea virens[J]. Chinese Journal OF Rice Science, 2014
, 28(1)
: 9
-14
.
DOI: 10.3969/j.issn.1001-7216.2014.01.002
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