Effect of feeding of brown planthopper (BPH), Nilaparvata lugens (Stl), on depositional site, shape and numbers of callose in various leafsheath tissues were investigated using frozen section and fluoresence microscopy. The results showed that callose was mainly deposited in phloem, parenchyma, epidermal and xylem tissues, with phloem being dominant. Most calloses have a small granular shape. After treated with BPH feeding, manual acupuncture or injection of BPH saliva, numbers and distribution of callose in leafsheath increased evidently in each of the three rice varieties that have different tolerance, in particular in the susceptible variety TN1. As compared with healthy plants (control), after eight days recovery growth from BPH removal, callose number and distribution did not change significantly in TN1 leafsheaths, reduced evidently in the tolerant variety Utri Rajapan, and reduced slightly in the antibiosis variety Rathu Heenati. By contract, in the treatments of manual acupuncture or injection of BPH saliva, numbers and distribution of callose after eight days of recovery growth were significantly increased in the susceptible variety, changed little in tolerant variety, and increased slightly in antibiosis variety, as compared with control plants. It was concluded that tolerance of rice varieties were related to abundance of callose deposited in phloem tissue of leafsheath.
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