Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2013 , Vol. 27 >Issue 6: 559 - 568

DOI: https://doi.org/10.3969/j.issn.1001-7216.2013.06.001

Research Papers

Study on  the Genetic Basis for  Abnormal Heading  in Hybrid Rice

Expand
  • 1 College of Agronomy, Anhui Agricultural University, Hefei 230036,China; 2 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081,China; 3  Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China;

Received date: 2013-05-03

  Revised date: 2013-07-12

  Online published: 2013-11-10

Fold

Abstract

The problem of  abnormal heading in hybrid rice caused great economic losses in recent years. The genetic basis of this phenomenon is still ambiguous and not documented up to now. Four testcrossing populations were constructed using 38 introgression lines (ILs) from Shuhui 527/Fuhui 838 population as male parents and four male sterile lines (II32A, XieqingzaoA, Gang 46A and Jin 23A) as female parents. The phenotypic evaluation was conducted in Hefei, Hangzhou and  Guangzhou in late season. QTL associated with photosensitivity (PS) was identified using oneway analysis of variance (ANOVA) and twoway ANOVA methods with the genotypic and phenotypic data. Progeny testing showed that 55 combinations did not head in Hefei, but normally headed with delayed heading date in Hangzhou and Guangzhou. A total of 12 QTL for PS was found in more than two environments or populations. Using twoway ANOVA, 31 epistatic QTLs affecting PS were detected in Hangzhou and Guangzhou, five of which were stably expressed in different populations and environments. The loci interaction between RM331RM2, RM331RM5346, RM3395RM16 and RM3325RM53 played an important role in controlling the formation of Daqingke for combinations from testcrossing WD57, WD71, WD77, WD78, WD80, WD85 and WD88 with fourtypes of male sterile lines.

Key words： hybrid rice; abnormal heading; heading date; photosensitivity; QTL mapping

Cite this article

QU Lijun1,2, ZHANG Hongjun2, XIANG Chao2, WANG Hui3, XIA Jiafa3, LI Zefu3, GAO Yongming2,*, SHI Yingyao1,* . Study on  the Genetic Basis for  Abnormal Heading  in Hybrid Rice[J]. Chinese Journal OF Rice Science, 2013 , 27(6) : 559 -568 . DOI: 10.3969/j.issn.1001-7216.2013.06.001

References

\[1\]Cheng S H, Zhuang J Y, Fan Y Y, et al. Progress in research and development on hybrid rice: A  superdomesticate in China. Ann Bot,  2007, 100(5): 959966.

\[2\]李开平, 康艳琼.大青棵在杂交稻田中的发生特点与防控对策. 云南农业科技, 2010, 29(4): 5254.

\[3\]Taeko S, Yasunori N, Nozomi O, et al. Genetic interactions involves in the inhibition of heading by heading date QTL, Hd2 in rice under longday conditions. Theor Appl Genet,  2011, 123: 11331143.

\[4\]Xue W, Xing Y, Weng X, et al. Natural variation in  Ghd7  is an important regulator of heading date and yield potential in rice. Nat Genet,  2008, 40: 761767.

\[5\]Yan W H, Wang P, Chen H X, et al. A major QTL,  Ghd8,    plays pleiotropic roles in regulating grain productivity, plant height, and heading date in rice. Mol Plant, 2011, 4: 319330.

\[6\]Wei X, Xu J, Guo H, et al.  DTH8  suppresses flowering in rice, influencing plant height and yield potential simultaneously. Plant Physiol, 2010, 153: 17471758.

\[7\]Yano M, Katayose Y, Ashikari M, et al.  Hd1,    a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell, 2000, 12(12): 24732483.

\[8\]Kojima S, Takahashi Y, Kobayashi Y, et al.  Hd3a,    a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering down stream of  Hd1  under shortday conditions. Plant Cell Physiol,  2002, 43(10): 10961105.

\[9\]Takahashi Y, Shomura A, Sasaki T, et al.  Hd6,    a rice quantitative trait locus involved in photoperiod sensitivity, encodes the α subunit of protein kinase CK2. Proc Natl Acad Sci USA, 2001, 98(14): 79227927.

\[10\]Doi K, Izawa T, Fuse T, et al.  Ehd1,    a Btype response regulator in rice, confers shortday promotion of flowering and controls FTlike gene expression. Genes Dev, 2004, 18: 926936.

\[11\]Matsubara K, Yamanouchi U, Wang Z X, et al.  Ehd2,    a rice ortholog of the maize  INDETERMI NATE1  gene, promotes flowering by upregulating  Ehd1 . Plant Physiol,  2008, 148: 14251435.

\[12\]Dai C， Xue H W. Rice  early flowering1,    a CKI, phosphory lates DELLA protein SLR1 to negatively regulate gibberellin signalling. EMBO J,  2010, 29: 19161927.

\[13\]Komiya R, Ikegami A, Tamaki S, et al. Hd3a  and  RFT1  are essential for flowering in rice. Development,  2008, 135: 767774.

\[14\]Izawa T, Oikawa T, Tokutomi S, et al. Phytochromes confer the photoperiodic control of flowering in rice(a shortday plant). Plant J,  2000, 22(5): 391399.

\[15\]Lim J, Moon Y H, An G, et al. Two rice MADS domain proteins interact with  OsMADS1. Plant Mol Biol,  2000, 44: 513527.

\[16\]Kikuchi S, Satoh K, Nagata T, et al. Collection, mapping, and annotation of over 28, 000 cDNA clones from japonica rice. Science,  2003, 301 (5631)： 376379.

\[17\]Shinozuka Y, Kojima S, Shomura A, et al. Isolation and characterization of rice MADS box gene homologues and their RFLP mapping. DNA Res, 1999, 6 (2)： 123129.

\[18\]Lee S, Kim J, Han J J, et al. Functional analyses of the flowering time gene  OsMADS50,    the putative  SUPP RESSOR OF OVEREXPRESSION OF CO1/AGAMOUSLIKE20  ( SOC1/AGL20 ) ortholog in rice. Plant J,  2004, 38(5): 754764.

\[19\]Kim S L, Lee S, Kim H J, et al.  OsMADS51  is a shortday flowering promoter that function supstream of  Ehd1 , OsMADS14,    and  Hd3a . Plant Physiol,  2007, 145: 14841494.

\[20\]Ryu C H, Lee S, Cho L H, et al. OsMADS50 and  OsMADS56  function antagonistically in regulating long day (LD) dependent flowering in rice. Plant Cell Environ,  2009, 32: 14121427.

\[21\]Hayama R, Izawa T, Shimamoto K, et al. Isolation of rice genes possibly involved in the photoperiodic control of flowering by a fluorescent differential display method. Plant Cell Physiol,  2002, 43(5): 494504.

\[22\]Li D, Yang C, Li X, et al. Functional characterization of rice  OsDof12.   Planta, 2009, 229: 1159 1169.

\[23\]Kim S K, Yun C H, Lee J H, et al.  OsCO3,  a CONSTANS LIKE gene, controls flowering by negatively regulating the expression of FTlike gene sunder SD conditions in rice. Planta,  2008, 228: 355365.

\[24\]Zhang H J, Wang H, Qian Y, et al. Simultaneous improvement and genetic dissection of grain yield and its related traits in a backbone parent of hybrid rice (Oryza sativa L.) using selective introgression. Mol Breeding， 2013, 31: 181194.

\[25\]Moncada P, Martinez C P, Borrero J, et al. Quantitative trait loci for yield and yield components in an Oryza sativaOryza rufipogon BC2F2 population evaluated in an upland environment. Theor  Appl Genet,  2001, 102: 4152.

\[26\]SAS Institute Inc.   SAS/STAT V9.2  User’s  Guide. SAS Institute Inc, Cary, NC, USA, 2008： 24302611.

\[27\]Thomson M J, Tai T H, McClung A M, et al. Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson. Theor Appl Genet, 2003, 107: 479493.

\[28\]陆作楣, 马崇云. 杂交稻的杂株与对策. 种子, 1987（27）: 38, 39.

\[29\]白和盛, 王宝和. 杂交稻青棵的成因及对策. 种子世界, 1991 （9）: 35.

\[30\]水清. 稻田里的“大青棵”从哪里来的. 江苏农业科技报, 2008（1）: 30.

\[31\]戴剑. 杂交稻亲本SSR指纹图谱构建及两系杂交稻和大青棵鉴定的研究\[D\]. 南京: 南京农业大学, 2011.

\[32\]于振文. 作物栽培学各论.  北京： 中国农业出版社, 2003: 154155.

\[33\]南京农学院. 作物栽培学. 上海： 上海科学技术出版社, 1979: 3435.
Options
Outlines

/

〈 〉

Tel: 0571-63370278　E-mail: cjrs@263.net
Supported by Beijing Magtech Co., Ltd.