To improve the efficiency of selection for Wxmq gene in breeding, we designed specific primers by tetraprimer Amplification Refractory Mutation System PCR (ARMSPCR) method, according to the single nucleotide mutation in Wxmq gene to analyze 12 rice varieties (or lines) and the F2 segregation population derived from the cross of lowamylose content variety Kanto 194 (with Wxmq gene) and normal variety Wuyujing 3(without Wxmq gene). The results of PCR detection indicated that three genotypes including the homozygous genotype with Wxmq, the homozygous genotype without Wxmq and the heterozygous genotype could be clearly distinguished according to band types, and it was completely consistent with the result of phenotypic analysis. Therefore, as a simple and lowcost technique，the tetraprimer ARMSPCR could be widely used to identify rice resources with Wxmq gene and applied in molecular markerassisted breeding.
CHEN Tao， LUO Mingrui， ZHANG Yadong， ZHU Zhen， ZHAO Ling， ZHAO Qingyong， ZHOU Lihui， YAO Shu， YU Xin， WANG Cailin*
. Detection of Wxmq Gene for Lowamylose Content by Tetraprimer Amplification Refractory Mutation System PCR in Rice[J]. Chinese Journal OF Rice Science, 2013
, 27(5)
: 529
-534
.
DOI: 10.3969/j.issn.1001-7216.2013.05.010
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