To explore the relationship between auxin efflux protein OsPIN1a and negative phototropism of rice roots, the complete open reading frame (ORF) of OsPIN1a was amplified based on the sequence deposited in GenBank by RTPCR with genespecific primers. Sequencing results show that the GC content of OsPIN1a ORF is 65.49%. The fusion expression vector pCAMBIA1301OsPIN1a::GFP containing OsPIN1a and a coding green fluorescent protein (gfp) gene was constructed and transferred into onion skin cells by the Agrobacterium tumefaciens transformation. Transient expression of the OsPIN1aGFP protein showed that the protein was mainly located on the nucleus and cell membrane. Genetic transformation of japonica rice variety Zhonghua 11 was mediated by Agrobacterium tumefaciens. Molecular detection of transformed rice plants by PCR and GUS staining showed that the target construct was integrated into the genome of rice. The negative phototropism curvature of the transformed rice root were higher than that of the wild type as well as the expression level of OsPIN1a in transformed plants, indicating that OsPIN1a probably played an important role in negative phototropism curvature of rice root.
XU Huawei1, MO Yiwei2, 3, *, SHI Guoan1, JIN Wenlong2, WANG Zhong4
. Preliminary Study on Function of OsPIN1a Gene in Negative Phototropism of Rice Roots[J]. Chinese Journal OF Rice Science, 2013
, 27(5)
: 466
-472
.
DOI: 10.3969/j.issn.1001-7216.2013.05.003
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