An oligidic diet for larvae rearing of the pink stem borer (PSB), Sesamia inferens (Walker), was optimized by using the uniform design. The formulation of the optimized diet was as follows: agar powder 3 g, soybean powder 8 g, water bamboo powder 11 g, yeast powder 16 g, sucrose 6 g, rice powder 3 g, corn powder 11 g, wheat germ powder 5 g, casein 7 g, vitamin C 0.8 g, sorbic acid 0.64 g, aureomycin 0.54 g, cholesterol 0.04 g, Wesson’s salt 15 g, and distilled water 200 g. PSB had been reared on the diet for more than ten successive genenrations, much more than that previously reported. The rate of the 1st instar PSB larvae that developed to pupae on the optimized diet reached 87.5%, significantly higher than that on the benchmark diet. As for the ten generations’ succesive rearing, the average of pupation rate, emergence rate, egg hatching rate and fecundity of PSB on the optimized diet was 42.5%-87.5%, 79.7%-95.8%, 90.0%-94.9% and 95-252 eggs per female, respectively. There was no significant difference between the 10th genenation and the 1st of PSB reared on the diet, except the significantly longer male larval stage.
DAI Changgeng1,2， LI Kailong2， WANG Lifeng1， TAN Xiansheng1， HU Yang2， YUAN Zheming1，*， FU Qiang1,2,*
. An Oligidic Diet for Sesamia inferens Optimized by Uniform Design and Successive Rearing[J]. Chinese Journal OF Rice Science, 2013
, 27(4)
: 434
-439
.
DOI: 10.3969/j.issn.1001-7216.2013.04.014
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