To promote the scientific utilization of M. oryzae databases, the location and length of functional genes, the distribution and number of SSR loci, SSR primer’s location, and other characteristics in four M. oryzae databases and Chr7 BAC sequence database were compared. The results suggest that the length and annotated gene numbers, location of functional genes, SSR numbers of nucleotide repeats and SSR primer location in the four M. oryzae databases and Chr7 BAC sequence database differ. AvrPizt linked marker location assay shows that genome sequences in different databases have significant differences. Our results also indicate significant differences among the four M. oryzae databases and Chr7 BAC sequence database. Though the difference among different databases exerting not effect on the gene homolog clone, it affects the map based cloning of avirulence gene. In a word, researchers need to choose which database can be used in their studies according to the purpose.
MAO Xueqin, JIANG Hua, WANG Yanli, ZHANG Zhen, CHAI Rongyao, WANG Jiaoyu, QIU Haiping, DU Xinfa, SUN Guochang*
. Comparison on Genome Sequence of Magnaporthe oryzae in Different Assembly Databases[J]. Chinese Journal OF Rice Science, 2013
, 27(4)
: 425
-433
.
DOI: 10.3969/j.issn.1001-7216.2013.04.013
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