Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2013 , Vol. 27 >Issue 4: 381 - 388

DOI: https://doi.org/10.3969/j.issn.1001-7216.2013.04.007

Research Papers

Characterization of Cold Tolerance  and Identification of Cold Tolerance QTLs for Rice Single Segment Substitution Lines at Plumule  and Seedling Stages

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  • 1 Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China; 2 Leshan Agricultural Science Research Academy, Leshan 614000, China;

Received date: 2013-02-20

  Revised date: 2013-03-19

  Online published: 2013-07-10

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Abstract

The cold tolerance at plumule  stage (CTP) and seedling stage (CTS) were respectively evaluated at 4℃ and 13℃/7℃ (day/night) using a set of single segment substitution lines (SSSLs) derived from indica recipient (9311) and japonica donor (Nipponbare). The survival seedling rate and the normal leaf rate (NLR) were used as the estimation index for CTP and CTS, respectively. The results showed that the CTP and CTS of Nipponbare were extremely stronger than those of  9311, and the difference of CTP and CTS were extremely significant among SSSLs. The SSSLs (X724, X732 and X733) with strong CTP and CTS mostly kept the agronomic traits of 9311. The General Combing Ability (GCA) of these three SSSLs, such as yield GCA, were much better than  that of 9311. A total of 18 quantitative trait loci (QTLs) for CTP, 5 QTLs for CTS were identified among 20 introgression segments distributed on 12 chromosomes. Among these QTLs, the CTP QTLs (qCTP9, qCTP11.2  and qCTP12.1), and the CTS QTLs (qCTS1.1 and qCTS1.2) showed more additive effect, and the corresponding SSSLs of those QTLs displayed stronger CTP and CTS, respectively.

Key words： rice; single segment substitution lines; plumule stage; seedling stage; cold tolerance; QTL

Cite this article

ZHOU Yong1，2 , ZHU Xiaobo１， YUAN Hua1， ZHENG Ying2， QIN Peng１， WEI Yinghai1，2， WANG Yuping1， HUANG Shijun2， LI Shigui1，* . Characterization of Cold Tolerance  and Identification of Cold Tolerance QTLs for Rice Single Segment Substitution Lines at Plumule  and Seedling Stages[J]. Chinese Journal OF Rice Science, 2013 , 27(4) : 381 -388 . DOI: 10.3969/j.issn.1001-7216.2013.04.007

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