Genetic Variation Analysis of Paddy Rice Landrace of&nbsp; Yuelianggu from Yuanyang Hani&rsquo;s Terraced Fields in Yunnan Province

DONG Chao1,#, XU Furong1,#, YANG Wenyi1, TANG Cuifeng1, ZHANG Enlai1 , YANG Yayun1, A Xinxiang1, ZHANG Feifei1 , LU Guangde2, WANG Yan2, DAI  Luyuan1, *

doi:10.3969/j.issn.10017216.2013.02.005

Chinese Journal OF Rice Science >

2013 , Vol. 27 >Issue 2: 137 - 144

DOI: https://doi.org/10.3969/j.issn.10017216.2013.02.005

Research Papers

Genetic Variation Analysis of Paddy Rice Landrace of Yuelianggu from Yuanyang Hani’s Terraced Fields in Yunnan Province

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¹Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences /Yunnan Provincial Key Laboratory of Agricultural Biotechnology/ Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture/ Scientific Observation for Rice Germplasm Resources of Yunnan, Ministry of Agriculture, Kunming 650223, China; ²Yuanyang Department of Agriculture,Yuanyang 662414, China;

Received date: 2012-05-02

Revised date: 2012-08-21

Online published: 2013-03-10

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Abstract

In order to reveal the genetic variation of rice landrace Yuelianggu which has been widely grown in Yunnan Hani’s terraced fields for longterm,98 Yuelianggu samples collected from 11 villages located in Yunnan and 8 rice varieties as control were analyzed by using 20 phenotypic traits and 48 SSR markers. The results showed that the variation coefficients for the quantitative characters were larger than those of the qualitative characters with a range from 2.55% to 24.90%. A total of 114 alleles in all of Yuelianggu were found with the mean of 2.38 and range from 1 to 4, as well as the number of 87.65 effective alleles with the mean of 1.83 and range from 1 to 3.02. The mean of Nei’s genetic diversity index (I) was 0.64. Clustering analysis based on phenotypes could divide the used materials including Yuelianggu and control varieties into two categories, with Yuelianggu belonging to one group while the other eight varieties to another one. However, clustering analysis based on SSR markers could divide the tested moterials into two categories, with one group including all Yuelianggu samples and the indica control while another one only including the japonica control. Analysis of molecular variance （AMVOA） revealed that the intervillage genetic variations of Yuelianggu consisted of 67.18% and the intravillage genetic variation contisted of 37.82%. Yuelianggu samples collected from 11 villages could be divided into 5 populations, which were closely related to geographical distance between the villages. Genetic distances of Yuelianggu collected from adjacent villages were small while their genetic similarities were high correspondly. The special genetic variation of Yuelianggu resulted from the unique Hani’s terraced fields and its traditional culture for longterm. Yuelianggu can be used as a benefit gene resource to further exploit and utilize.

Key words： Hani’s terraced field; rice; genetic variation; Yuelianggu

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DONG Chao1,#, XU Furong1,#, YANG Wenyi1, TANG Cuifeng1, ZHANG Enlai1 , YANG Yayun1, A Xinxiang1, ZHANG Feifei1 , LU Guangde2, WANG Yan2, DAI Luyuan1, * . Genetic Variation Analysis of Paddy Rice Landrace of Yuelianggu from Yuanyang Hani’s Terraced Fields in Yunnan Province[J]. Chinese Journal OF Rice Science, 2013 , 27(2) : 137 -144 . DOI: 10.3969/j.issn.10017216.2013.02.005

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