Identification and Gene Cloning of the Leafy Head Mutant pla15 in Rice

FENG Gongneng1,2, ZHANG Changquan1, ZHAO Dongsheng1, ZHU Kongzhi1, TU Huaizhou1, XU Chenwu1, LIU Qiaoquan1,*

doi:10.3969/j.issn.10017216.2013.02.001

Chinese Journal OF Rice Science >

2013 , Vol. 27 >Issue 2: 111 - 116

DOI: https://doi.org/10.3969/j.issn.10017216.2013.02.001

Research Papers

Identification and Gene Cloning of the Leafy Head Mutant pla15 in Rice

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¹Key Laboratory of Plant Functional Genomics of the Ministry of Education/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Agriculture, Yangzhou University, Yangzhou 225009, China; ² Yancheng Institute of Technology, Yancheng 224051, China;

Received date: 2012-07-30

Revised date: 2012-09-20

Online published: 2013-03-10

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Abstract

From the progeny of a japonica variety Taipei 309 treated with 60Coγ ray irradiation, a leafy head mutant pla15 (plasttochron 15) with dwarf phenotype and small leaf was identified. Comparing with the wild type, the pla15 mutant has more leaves and less tillers, and therefore fails to produce a normal panicle at the maturity stage. The genetic analysis showed that the pla15 phenotype was controlled by a single recessive nuclear gene. By the mapbased cloning, the target gene was finally narrowed down to a 58 kb region between SSR markers CHR1027 and CHR1030 on the long arm of chromosome 10, and cosegregates with the molecular markers CHR1028 and CHR1029. There were five predicted genes in the mapped region. The sequencing analysis results revealed that there was a deletion of the nucleotide T in the first exon of the gene LOC_Os10g26340 encoding cytochrome P450 CYP78A11 in the pla15 mutant, which might result in a downstream frame shift and a premature termination. These results implied that the P450 CYP78A11 gene might be the candidate gene of PLA15.

Key words： Oryza sativa L.; leafy head mutant pla15; genetic analysis; gene cloning; P450 CYP78A11

Cite this article

FENG Gongneng1,2, ZHANG Changquan1, ZHAO Dongsheng1, ZHU Kongzhi1, TU Huaizhou1, XU Chenwu1, LIU Qiaoquan1,* . Identification and Gene Cloning of the Leafy Head Mutant pla15 in Rice[J]. Chinese Journal OF Rice Science, 2013 , 27(2) : 111 -116 . DOI: 10.3969/j.issn.10017216.2013.02.001

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