Research Papers

Characterization and Gene Mapping of a Dwarf Mutant Zj88d in Rice

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  • 1 College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310036, China; 2 Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 3 College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China;

Received date: 2012-03-13

  Revised date: 2012-08-22

  Online published: 2013-01-10

Abstract

The stably inherited dwarf mutant Zj88d was produced by treating the dry seeds of japonica rice variety Zhejing 88 with ethyl methylsulfonate (EMS). Genetic analysis of the dwarf mutant Zj88d revealed that this trait is controlled by one pair of recessive nuclear gene.  The gene was located in the interval of 603 kb between   744w and RM5344 markers on the short arm of chromosome 7 by mapbased cloning. The dwarf gene “OSH15” was identified by screening one hundred candidate genes on the target chromosomal segment. Sequencing results further revealed that Zj88d gene has a singlebase substitution (g to a) in the last nucleotide of intron 2 of  OSH15 allele.

Cite this article

JI Xianjun1,2, YE Shenghai2, ZHOU Ya2,3, XIU Fenlian2,3, DENG Xiaomei1,2, SHANG Haixuan2,3, LIU Jiyun2,3, CHEN Pingping2,3, LI Xiaohua1,2, JIN Qingsheng2, ZHANG Xiaoming2,* . Characterization and Gene Mapping of a Dwarf Mutant Zj88d in Rice[J]. Chinese Journal OF Rice Science, 2013 , 27(1) : 35 -40 . DOI: 10.3969/j.issn.10017216.2013.01.005

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