The function of a rice zincfinger protein gene (OsZRL) was investigated by RNA interference. The results of semiquantitative RTPCR and quantitative PCR analysis indicated that OsZRL expression levels were significantly downregulated in the transgenic plants. The OsZRLRNAi transgenic lines were characterized by bigger leaves, stronger roots and stems compared to wild type plants, indicating that the suppression of OsZRL had a positive effect on rice growth. The expression pattern and transgenic seedlings phenotypes revealed that OsZRL was involved in gibberellin or abscisic acid signaling pathway. It suggests that zincfinger protein OsZRL might perform its function of negetive regulator in rice growth through the regulation of gibberellin and abscisic acid.
YU Xiaohong1, CHAO Li1, CHEN Changdong1, LIU Yongsheng1, 2, NIU Xiangli 1, 2, *
. Function Analysis of a Rice Zinc Finger Protein Gene OsZRL by RNA Interference[J]. Chinese Journal OF Rice Science, 2013
, 27(1)
: 9
-16
.
DOI: 10.3969/j.issn.10017216.2013.01.002
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