Forty SSILP, 30 InDel and 43 SSR markers were used to compare genetic diversity of 42 weedy and cultivated rice entries from Sri Lanka, 9 indica and 7 japonica rice cultivars as well as four representatives of weedy rice from China and other countries(regions). There were 84, 61 and 213 alleles identified by SSILP, InDel and SSR markers, with average polymorphism information content (PIC) values 0.257, 0.255 and 0.543, respectively. The NJ clusters analysis indicated all 42 Sri Lanka weedy and cultivated rice entries belonged to indica type. The genetic distances of SSILP and InDel markers revealed highly correlation (r=0.989). Meanwhile the associations between SSR markers and the other two markers (SSILP and InDel) were lower as 0.812 and 0.808, respectively. With the rising reliability and accuracy of indicajaponica classification resulted from the development of more subspeciesspecific markers, SSILP and InDel markers can be applied extensively for the indicajaponica classification of rice germplasm. And SSR markers can classify better in the subspecies.
XU Qun1 , XU Hongyun2 , WEI Xinghua1,* , TANG Shengxiang1 , LEE Dongsun2 , CHEN Lijuan2
. SSILP, InDel and SSR Markersbased indicajaponica Classification for Weedy Rice[J]. Chinese Journal OF Rice Science, 2012
, 26(6)
: 686
-692
.
DOI: 10.3969/j.issn.10017216.2012.06.008
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