The existence of the two genes of Pita and Pib in 176 rice landraces from Yunnan Province were rapidly determined using the dominant DNA markers of Pita/pita and Pib/pib alleles(YL155/YL18 and YL183/YL87 for the former, PibdomF/Pib dom R and Lys14SF/Lys145R for the latter designed according to their difference in sequence). The results showed that Pita was found in 72 among 176 landraces with the frequency of 40.9%. Among them, Pita was found in 44 among 77 indica and 28 among 99 japonica cultivars, the frequency in indica and japonica were 57.1% and 28.3%, respectively. The distribution frequency of the resistance gene Pita in the japonica and indica varieties significantly differed, the frequency was higher in indica than in japonica cultivars. While Pib was found in 33 among 176 landraces with the frequency of 18.8%. Among them, Pib was found in 15 indica and 18 japonica cultivars, the frequency in indica and japonica were 19.5% and 18.2%, respectively. Pita was mainly distributed in Yuxi, Baoshan, Lincang and Simao prefectures, while Pib was mainly distributed in Nujiang, Baoshan and Zhaotong prefectures of Yunnan Province. The results of pathogenic assays with the rice blast fungus in greenhouse were generally consistent with the results of molecular identification.
LI Jinbin1, WANG Tian2, XU Minghui2,*
. Identification of Pita and Pib Genes for Rice Blast Resistance of Rice Landraces from Yunnan Province[J]. Chinese Journal OF Rice Science, 2012
, 26(5)
: 592
-598
.
DOI: 10.3969/j.issn.10017216.2012.05.012
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