The second open reading frame on the genome segment 5 (S5) of Rice blackstreaked dwarf virus (RBSDV) encodes a viral nonstructural protein (p5b) with unknown function. To reveal the function of p5b, its gene was ligated into bait plasmid pGBKT7 and an expression library containing rice cDNAs was constructed using plasmid pGADT7 of yeast twohybrid. The bait protein p5b was detected in yeast by western blot and the result of an autoactivation test showed that p5b could not autonomously activate the expression of reporter genes in yeast. Then the bait protein p5b was used for screening the expression cDNA library of rice plant. Gene fragments of some pivotal enzymes, involved in photosynthesis, respiration, and other important metabolism processes, were identified to interact with p5b in yeast, suggesting that these interactions may play roles in symptom development of infected plants.
LU Ying1,2, YANG Jian2, ZHANG Hengmu2, CHEN Jianping2,*
. Screening of Rice Genes Interacted with p5b of Rice blackstreaked dwarf virus[J]. Chinese Journal OF Rice Science, 2012
, 26(5)
: 537
-541
.
DOI: 10.3969/j.issn.10017216.2012.05.004
\[1\]阮义理, 蒋文烈, 林瑞芬. 水稻黑条矮缩病的研究. 浙江农业科学, 1984(4): 185192.
\[2\]陈声祥. 水稻病毒病发生现状及研究进展. 浙江农业科学, 1996(1): 4142.
\[3\]Zhang H M, Chen J P, Lei J L, et al. Sequence analysis shows a dwarf disease on rice, wheat and maize in China is caused by rice blackstreaked dwarf virus. Euro J Plant Pathol, 2001, 107(5): 563567.
\[4\]Fauquet C M, Mayo M A, Maniloff J, et al. Virus taxonomy: Classification and nomenclature of viruses//Eighth Report of the International Committee on the Taxonomy of Viruses. San Diego: Elsevier Academic Press, 2005: 534542.
\[5\]Zhang H M, Chen J P, Adams M J. Molecular characterisation of segments 1 to 6 of Rice blackstreaked dwarf virus from China provides the complete genome. Arch Virol, 2001, 146: 23312339.
\[6\]张恒木. 水稻黑条矮缩病毒的分子生物学. 杭州: 浙江大学, 2001.
\[7\]Sambrook J, Russell D. Molecular Cloning: A Laboratory Manual. 3rd ed. New York：Cold Spring Harbor laboratory Press， 2001.
\[8\]Zhou F, Wu G, Deng W, et al. Interaction of rice dwarf virus outer capsid P8 protein with rice glycolate oxidase mediates relocalization of P8. FEBS Lett， 2007, 581(1): 3440.
\[9\]Zhu S, Gao F, Cao X, et al.The rice dwarf virus P2 protein interacts with entkaurene oxidases in vivo, leading to reduced biosynthesis of gibberellins and rice dwarf symptoms. Plant Physiol, 2005, 139(4): 19351945.
\[10\]吕明芳， 羊健， 张恒木， 等. 水稻黑条矮缩病毒基因组S7编码的2个非结构蛋白在病株中的表达检测. 中国水稻科学，2012， 25(1): 915.
\[11\]贾永光, 张立军, 刘淳, 等. 丙酮酸磷酸双激酶及其基因结构. 植物生理学通讯, 2009, 45(3): 305311.
\[12\]Kang H G, Park S, Matsuoka M, An G. Whitecore endosperm floury endosperm4 in rice is generated by knockout mutations in the C4type private orthophosphate dikinase gene (OsPPDKB). Plant J, 2005, 42: 901911.
\[13\]Moons A, Valcke R, Montagu VM. Lowoxygen stress and water deficit induce cytosolic pyruvate orthophosphate dikinase (PPDK) expression in roots of rice, a C3 plant. Plant J, 1998, 15(1): 8998.
\[14\]Bykova N, Keerberg O, Parnik T, et al. Interaction between photorespiration and respiration in transgenic potato plants with antisense reduction in glycine decarboxylase. Planta, 2005, 222: 130140.
\[15\]张国, 王玮, 邹琦. Rubisco活化酶的分子生物学. 植物生理学通讯, 2004, 40(5): 633637.
\[16\]Ristic Z, Momcilovic I, Budovinik U, et al. Rubisco activase and wheat productivity under heatstress conditions. J Exp Bot, 2009, 60: 40034014.
\[17\] Green P J. The ribonucleases of higher plants. Annu Rev Plant Physiol Plant Mol Biol, 1994, 45: 421445.
\[18\]聂明建, 王国槐. 植物核糖核酸酶与转基因工程雄性不育系研究进展. 中国农学通报, 2006, 22(2): 122127.
\[19\]Kruger J, Thomas C M, Golstein C, et al. A tomato cysteine protease required for Cf2dependent disease resistance and suppression of autonecrosis. Science, 2002, 296: 744747.
\[20\]Mohan S, Ma P K W, Pechan T, et al. Degradation of the Spodoptera frugiperda peritrophic matrix by an inducible maize cysteine protease. J Insect Physiol, 2005, 52: 2128.
\[21\]孙卫红, 王伟青. 植物抗坏血酸过氧化物酶的作用机制酶学及分子特性. 植物生理学通讯, 2005, 41（2）: 143147.
\[22\]Willekens H, Chamnongpol S, Davey M, et al. Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants. EMBO J， 1997, 16: 48064816.
\[23\] Yang Q, Reinhard K, Schiltz E, et al. Characterization and heterologous expression of hydroxylcinnamoyl/benzoylCoA: Anthranilate Nhydroxycinnamoyl/benzoyltransferase from elicited cell cultures of carnation, Dianthus caryophyllus L. Plant Mol Biol, 1997, 35: 777789.
