Research Papers

Differential Protein Expression  Analyses of  Pollens of  Honglian CMS Rice at Uninucleate Stage

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  • 1 Chemical and Biological Engineering College, Changsha University of Science and Technology, Changsha 410114, China; 2 Key Laboratory of MOE for Plant Developmental Biology, College of Life Science, Wuhan University, Wuhan 430072, China; 3 Institute of Oilseed Crops, Hunan Agricultural University, Changsha 410128, China;

Received date: 2012-01-09

  Revised date: 2012-02-04

  Online published: 2012-09-10

Abstract

To better understand the molecular mechanism of cytoplasmic male sterility (CMS) at the protein level during the development of microspores,   the protein expression profile  was analyzed in pollens of the CMS rice line  Yuetai A, its fertile maintainer line Yuetai B and their hybrid F1 (Honglianyou 6). The proteins were separated by two dimensional electrophesis  with immobilized pH (3-10 nonlinear, NL) gradients as the first dimension and SDSPAGE as the second, and 15 differentially expressed proteins were identified by Matrix assisted laserdesorption/ionizaton time of flight mass spectrometry (MALDITOF/MS) or liquid chromatographytandem mass spectrometry (LCMS/MS) analyses. Among these proteins,  some involved in substance and energy metabolism, cell cycle, transcription and cellular transporters etc. were downregulated or absent in the malesterile line, including a putative potassium/proton antiporterlike protein, a zinc knuckle domainlike, a WDrepeat protein etc.  It seemed  to suggest that the downregulation  or absence of these proteins in pollen might relate to the pollen sterility caused by the insufficient mitochondrial energy.

Cite this article

WEN Li1, 3,*, LIU Gai2, WANG Kun2, TAN Bijun1, TAO Jun1 . Differential Protein Expression  Analyses of  Pollens of  Honglian CMS Rice at Uninucleate Stage[J]. Chinese Journal OF Rice Science, 2012 , 26(5) : 529 -536 . DOI: 10.3969/j.issn.10017216.2012.05.003

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