Indicajaponica differentiation degree of 81 japonica cultivars belonging to different ecotypes from JiangHuai Region were investigated by 25 specific SSR markers of indicajaponica differentiation. TDj values of the cultivars ranged from 0.773 to 0.962, indicating most of the cultivars had a high japonica component degree with the medium maturing midseason japonica(MMMj) being the highest and late japonica being the lowest. In the 81 japonica cultivars, a total of 131 alleles were detected at 49 SSR loci, and allele number per marker ranged from 2 to 5, with an average of 2.67. Polymorphism information content(PIC) value of 49 SSR primer ranged from 0.068 to 0.657,with the average value 0.362. Among the 12 chromosomes，chromosome 11 showed the highest average allele number and PIC value. Of the three different ecological type japonica cultivars, genetic diversity of the late maturing midseason japonica(LMMj) was the highest according to PIC and mean heterozygosity per locus(He). The coefficient of genetic similarity was the highest while the value of genetic distance was the lowest between MMMj and LMMj, and the coefficient of genetic similarity was the lowest while the value of genetic distance was the highest between MMMj and late japonica. Clustering analysis with UPGMA method showed cultivars bred in the same breeding organization had a small average genetic distance among cultivars.
ZHOU Zhenling1,2 , WANG Baoxiang1, FAN Jiwei1, LU Baiguan1, ZHAO Zhigang2, JIANG Ling2, QIN Derong1, WAN Jianmin2,3, XU Dayong1,2,*
. Indicajaponica Differentiation Degree and Genetic Diversity of japonica Cultivars Belonging to Different Ecotypes from the YangtzeHuaihe Region[J]. Chinese Journal OF Rice Science, 2012
, 26(4)
: 431
-437
.
DOI: 10.3969/j.issn.10017216.2012.04.007
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