A realtime PCR quantitative detection technology was established by using two pairs of specific primers designed according to the internal transcribed spacer (ITS) sequences of Ustilaginoidea virensand their corresponding TaqMan probes. The results showed that the combination of the specific primers and probe of uvr292rf/uvr397rr/uvrp333 was highly sensitive and specific in the quantification of U. virens with a minimum detection limit of 24 fg. A linear model between Ct and common logarithm of spores number was established by realtime PCR with the templates of DNA from diluted spores solution and the theoretical detected limit was 0.67 spore. According to the quantitative detection of three air samples collected in paddy field at various time with this linear model, the quantity of this fungus conidia fluctuated monthly.
ZHENG Jing1，2， ZHANG Zhen2，*， JIANG Hua2， WANG Yanli2， CHAI Rongyao2， QIU Haiping2， MAO Xueqin2，WANG Jiaoyu2， DU Xinfa2， LAN Zhaohui3， SUN Guochang2，*
. Establishment and Preliminary Application of a Quantitative Realtime PCR Method for Detection of Conidia of Ustilaginoidea virens [J]. Chinese Journal OF Rice Science, 2012
, 26(4)
: 500
-505
.
DOI: 10.3969/j.issn.10017216.2012.04.017
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