A recombinant inbred population derived from the cross between a javonica(D50)and an indica(HB277)rice was used to investigate the genetic factors affecting plant height. A total of six quantitative trait loci(QTLs)with significant additive effect were identified for the plant height, and they were located on chromosome 1, 2, 3, 4, 6 and 8. One QTL qPH3 can stably express and have significant additive effects in Hangzhou, Zhejiang Province with the explained variance of 16.52% and Linshui, Hainan Province with the explained variance of 16.60%. One residual heterozygous line(RHL) was selected from the RILs and a RHLF2 population was constructed. The major QTL qPH3 with the explained variance of 76.13% was detected with the RHLF2 population. By progeny tests, qPH3 was finally mapped within about 204 kb in the region RM16211-RM16237. These results will be helpful for fine mapping and mapbased cloning of this QTL.
LUO Ju#, SHAO Gaoneng#, WEI Xiangjin, CHEN Mingliang, TANG Shaoqing, JIAO Guiai, XIE Lihong, HU Peisong*
. Genetic Analysis of a QTL qPH3 for Plant Height in Rice[J]. Chinese Journal OF Rice Science, 2012
, 26(4)
: 417
-422
.
DOI: 10.3969/j.issn.10017216.2012.04.005
\[1\]Li Z, Pinson S R M, Stansel J W, et al. Identification of two major genes and quantitative trait loci (QTLs) for heading date and plant height in cultivated rice (Oryza sativa L). Theor Appl Genet, 1995, 91: 374381.
\[2\]Khush G S. Green revolution: Preparing for the 21st century. Genome, 1999, 42(4): 646655.
\[3\]马良勇,包劲松,李西明,等.水稻矮生基因的克隆和功能研究进展.中国水稻科学, 2009, 23(1): 111.
\[4\]Ishimaru K, Ono K, Kashiwagi T, et al. Identification of a new gene controlling plant height in rice using the candidategene strategy. Planta, 2004, 218: 388395.
\[5\]鲍钱江, 樊叶杨, 於卫东, 等. 水稻第6染色体短臂上株高QTL qPH61的精细定位. 中国水稻科学, 2009, 23(5): 470474.
\[6\]Xue W Y, Xing Y Z, Weng X Y, et al. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice. Nat Genet, 2008, 40: 761767.
\[7\]YanW H, Wang P, Chen H X, et al. A major QTL, Ghd8, plays pleiotropic roles in regulating grain productivity, plant height, and heading date in rice. Mol Plant, 2011, 4(2): 319330.
\[8\]邵高能, 唐绍清, 焦桂爱, 等. 稻米蒸煮品质性状的QTL定位. 中国水稻科学, 2009, 23(1):9498.
\[9\]卢扬江,郑康乐. 一种简易提取水稻DNA的方法. 中国水稻科学, 1992, 6(1): 4748.
\[10\]Shao G N, Tang A, Tang S Q, et al. A new deletion mutation of fragrant gene and the development of three molecular markers for fragrance in rice. Plant Breed, 2011,130 (2), 172176.
\[11\]Lincoln S, Daley M, Lander E, et al.Constructing genetic maps with MAPMAKER/EXP 3.0//Whitehead Institute Technical Report.Cambridge MA,USA: Whitehead Institute, 1992.
\[12\]Wang S, Basten C J, Zeng Z B, et al.Win QTL Cartographer 2.5.Raleigh,USA:Department of Statistics,North Carolina State University, 2006.
\[13\]McCouch S R. Gene nomenclature system for rice. Rice, 2008, 1: 7284.
\[14\]孙永建, 周济, 徐华山, 等. 利用代换系分析水稻株高QTL及其互作效应. 分子植物育种, 2010, 8(6):10681073.
\[15\]Asano K, Yamasaki M, Takuno S, et al. Artificial selection for a green revolution gene during japonica rice domestication. PNAS, 2011, 108(27): 1103411039.
\[16\]Yamanaka N, Watanabe S, Toda K, et al. Fine mapping of the FT1 locus for soybean flowering time using a residual heterozygous line derived from a recombinant inbred line. Theor Appl Genet, 2005, 110: 634639.
\[17\]Yu S W, Yang C D, Fan Y Y, et al. Genetic dissection of a thousandgrain weight quantitative trait locus on rice chromosome. Chin Sci Bull, 2008, 53: 13891394.
\[18\]Shao G N, Tang S Q, Luo J, et al. Mapping of qGL72, a grain length QTL on chromosome 7 of rice. J Genet Genom, 2010, 37: 523531.
\[19\]Jiang G H, Xu C G, Li X H,et al. Characterization of the main effects, epistatic effects and their environmental interactions of QTL on the genetic basis of plant height and heading date in rice. Sci Agric Sin, 2005, 43: 161168.
\[20\]刘文俊, 王令强, 何予卿, 等. 利用2个相关群体定位和比较水稻株高与抽穗期QTL. 华中农业大学学报, 2007, 26: 161166.
\[21\]Oikawa T, Koshioka M, Kojima K, et al. A role of OsGA20ox1, encoding an isoform of gibberellin 20oxidase, for regulation of plant stature in rice. Plant Mol Biol, 2004, 55(5): 687700.