To reveal the relationship between endogenous dormancy mechanism of chlamydospores of U.virens and their walls, polysaccharide from cell walls of the dormant (black) and nondormant (yellow) chlamydospores was obtained by complex enzymehot water extractionSevag after the chlamydospores wall breaking through liquid nitrogen grinding plus ultrasonic crushing technique, and structure and composition of cell wall polysaccharide were analyzed by ultraviolet absorption spectrum (UV spectrum)，infrared absorption spectrum (IR spectrum), Congo red test, and gas chromatography (GC). The results showed that the polysaccharide from two kinds of the chlamydospores wall was acidic heteropolysaccharide and mainly consisted of the three monosaccharides, glucose, galactose and mannose, but the yellow chlamydospores wall still contained a small amount of xylose. Further, polysaccharide in the black chlamydospores wall, was composed of glucose, galactose, mannose with a molar percentage ratio of 58.23∶25.32∶16.46，while the yellow chlamydospore wall was composed of glucose, galactose, mannose and xylose, with a molar percentage ratio of 35.48∶30.58∶24.46∶9.45. The composition and composition ratios of monosaccharides in polysaccharide from the dormant and nondormant chlamydospore walls obviously varied, which may be closely related to chlamydospore dormancy.
WANG Na1, REN Zuohua1, DENG Linwei2, CHEN Juanfang3 , LIU Erming1，*
. Structure and Composition of Polysaccharide from Chlamydospore Wall in Ustiloginoidea virens[J]. Chinese Journal OF Rice Science, 2012
, 26(3)
: 356
-360
.
DOI: 10.3969/j.issn.10017216.2012.03.015
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