The changes in activities of key enzymes involved in sucrosetostarch conversion and contents of hormones in superior and inferior spikelets were investigated and their relation with grain filling of super rice was analyzed with four super rice cultivars, Liangyoupeijiu and Ⅱyou 084 (indica hybrids), Huaidao 9 and Wujing 15 (japonica), and two highyielding and elite check cultivars, Shanyou 63 (indica hybrid) and Yangfujing 8 (japonica)as material in paddy field. Activities of sucrose synthase (SuSase), adenosine diphosphoglucose pyrophosphorylase (AGPase), starch synthase (StSase), and starch branching enzyme (SBE) and contents of zeatin + zeatin riboside (Z+ZR), indole3acetic acid (IAA), abscisic acid (ABA) in superior and inferior spikelets were determined during the grain filling period and their relationships with grain filling rate were analyzed. Maximum grain filling rate, the time reaching a maximum grainfilling rate, mean grain filling rate and brown rice weight for superior spikelets slightly differed between super rice and elite check rice, but those for inferior spikelets were significantly different between super rice and check rice cultivars. Changes in enzyme activity and contents of hormones in grains followed single peak curves during the grain filling period. The peak and mean activity of SuSase, AGPase, StSase and SBE were lower in inferior spikelets than in superior ones,as well as the peak and mean contents of Z+ZR, IAA and ABA. The peak and mean contents of ABA were significantly greater in inferior spikelets than in superior ones and greater in the super rice than in the check rice. The grain filling rate was positively significantly correlated with the activities of SuSase, AGPase and StSase and contents of Z+ZR and IAA, but not significantly correlated with activity of SBE and ABA contents. The results suggested that the low activities of SuSase, AGPase and StSase and low contents of Z+ZR and IAA may be an important reason for the slow grain filling rate and low grain weight of inferior spikelets in super rice.
FU Jing, XU Yunji, CHEN Lu, YUAN Limin, WANG Zhiqin, YANG Jianchang*
. Postanthesis Changes in Activities of Enzymes Related to Starch Synthesis and Contents of Hormones in Superior and Inferior Spikelets and Their Relation with Grain Filling of Super Rice[J]. Chinese Journal OF Rice Science, 2012
, 26(3)
: 302
-310
.
DOI: 10.3969/j.issn.10017216.2012.03.008
\[1\]Kato T, Takeda K. Associations among characters related to yield sink capacity in spaceplanted rice. Crop Sci,1996, 36: 11351139.
\[2\]Kato T, Shinmura D, Taniguchi A. Activities of enzymes for sucrosestarch conversion in developing endosperm of rice and their association with grain filling in extraheavy panicle types.Plant Prod Sci, 2007, 10: 442450.
\[3\]Peng S B, Cassman K G, Virmani S S, et al. Yield potential trends of tropical since the release of IR8 and its challenge of increasing rice yield potential.Crop Sci, 1999, 39: 15521559.
\[4\]Cheng S H, Zhuang J Y, Fan Y Y, et al. Progress in research and development on hybrid rice: A superdomesticate in China. Ann Bot, 2007, 100: 959966.
\[5\]Peng S B, Khush G S, Virk P, et al. Progress in ideotype breeding to increase rice yield potential. Field Crops Res,2008, 108: 3238.
\[6\]敖和军, 王淑红, 邹应斌, 等. 超级杂交稻干物质生产特点与产量稳定性研究. 中国农业科学, 2008, 41(7): 19271936.
\[7\]Yang J C, Zhang J H. Grain filling problem in “super” rice. J Exp Bot,2010, 61: 15.
\[8\]Zhang H, Tan G L, Yang L N, et al. Hormones in the grains and roots in relation to postanthesis development of inferior and superior spikelets in japonica/indica hybrid rice. Plant Physiol Biochem,2009, 47: 195204.
\[9\]Mohapatra P K, Patel R, Sahu S K. Time of flowering affects grain quality and spikelet partitioning within the rice panicle.Aust J Plant Physiol, 1993, 20: 231242.
\[10\]Yang J C, Peng S B, Visperas R M, et al. Grain filling pattern and cytokinin content in the grains and roots of rice plants. Plant Growth Regul, 2000, 30: 261270.
\[11\]Liang J S, Zhang J H, Cao X Z. Grain sink strength may be related to the poor grain filling of indicajaponica rice (Oryza sativa) hybrids. Physiol Plantarum, 2001, 112: 470477.
\[12\]Yang J C, Zhang J H, Huang Z L, et al. Correlation of cytokinin levels in the endosperm and roots with cell number and cell division activity during endosperm development in rice. Ann Bot, 2002, 90: 369377.
\[13\]Nakamura Y, Yuki K. Changes in enzyme activities associated with carbohydrate metabolism during development of rice endosperm. Plant Sci, 1992, 82: 1520.
\[14\]Murata Y, Matsushima S. Rice//Evans L T, ed. Crop physiology. Cambridge: Cambridge University Press, 1975. 7599.
\[15\]Nakamura Y, Yuki K, Park S Y. Carbohydrate metabolism in the developing endosperm of rice grains. Plant Cell Physiol,1989, 30: 833839.
\[16\]Kato T. Change of sucrose synthase activity in developing endosperm of rice cultivars. Crop Sci,1995, 35: 827831.
\[17\]Ahmadi A, Baker D A. The effect of water stress on the activities of key regulatory enzymes of the sucrose to starch pathway in wheat. Plant Growth Regul, 2001, 35: 8191.
\[18\]Yang J C, Zhang J H, Wang Z Q, et al. Postanthesis development of inferior and superior spikelets in rice in relation to abscisic acid and ethylene.J Exp Bot, 2006, 57: 149160.
\[19\]Yang J C, Zhang J H, Wang Z Q, et al. Hormones in the grains in relation to sink strength and postanthesis development of spikelets in rice. Plant Growth Regul, 2003, 41: 185195.
\[20\]Richards F J. A flexible growth function for empirical use. J Exp Bot,1959, 10(29): 290300.
\[21\]朱庆森, 曹显祖, 骆亦其. 水稻籽粒灌浆的生长分析. 作物学报, 1988, 14(3): 182193.
\[22\]Yang J C, Zhang J H, Wang Z Q, et al. Activities of enzymes involved in sourcetostarch metabolism in rice grains subjected to water stress during filling. Field Crops Res, 2003, 81: 6981.
\[23\]程方民, 蒋德安, 吴平, 等. 早籼稻籽粒灌浆过程中淀粉合成酶的变化及温度效应特征. 作物学报, 2001, 27(2): 201206.
\[24\]陈远平, 杨文钰. 卵叶韭休眠芽中GA3、IAA、ABA和ZT的高效液相色谱法测定. 四川农业大学学报, 2005, 23(4): 498500.
\[25\]Yuan L P. Increasing yield potential in rice by exploitation of heterosis//Virmani S S.ed. Hybrid Rice Technology, New Developments and Future Prospects.Philippines: International Rice Research Institute, 1994: 16.
\[26\]Yuan L P. Hybrid rice breeding in China//Virmani S S, Sissiq E A, Muralidharan K.ed. Advances in Hybrid Rice Technology.Philippines: International Rice Research Institute, 1998: 2733.
\[27\]王志琴, 叶玉秀, 杨建昌, 等. 水稻灌浆期籽粒中蔗糖合成酶活性的变化与调节. 作物学报, 2004, 30(7): 634643.
\[28\]Preiss J. Biosynthesis of starch and its regulation//Preiss .ed. The Biochemistry of Plants. New York:Academic press 1988, 14: 181254.
\[29\]Okita T W. Is there an alternative pathway for starch synthesis?Plant physiol, 1992, 100: 560564.
\[30\]赵步洪, 张文杰, 常二华, 等. 水稻灌浆期籽粒中淀粉合成关键酶的活性变化及其与灌浆速率和蒸煮品质的关系. 中国农业科学, 2004, 37(8): 11231129.
\[31\]杨建昌, 王志琴, 朱庆森, 等. ABA与GA对水稻籽粒灌浆的调控. 作物学报, 1999, 25(3): 341348.
\[32\]Zhu G H, Ye N H, Yang J C, et al.Regulation of expression of starch synthesis genes by ethylene and ABA in relation to the development of rice inferior and superior spikelets. J Exp Bot, 2011, 62(11): 39073916.
