Research Papers

Molecular  Evolution and Expression Analysis of Subfamily ABCB Transporter Genes in Rice

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  • Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology; Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China;

Revised date: 2011-06-05

  Online published: 2012-03-10

Abstract

With the accomplishment of genome sequencing projects, over 130 ABCs were identified separately from the model plants of monocots and dicots, rice and Arabidopsis, but the functions of most of the members remain elusive.   Therefore, 27 and 29 subfamily B of ABC genes (ABCB) in rice and Arabidopsis were systematically characterized, respectively. The exon number,  length  and molecular weight  of proteins  encoded by these genes varied greatly  between the two plants, albeit the isoelectric point was less diverse.  Proteins of this subfamily  were divided into 4 subgroups accordingpar to   phylogenetic analysis, suggesting that divergence probably occurred among them; 6 and 9 pairs of paralogous genes were identified from rice and Arabidopsis, respectively, indicating that speciesspecific expansion contributed to the evolution of this subfamily in rice and Arabidopsis after the split of monocots and dicots. Segmental and tandem duplication contributed to the amplification of ABC family in rice. Multiple sequence alignment revealed that the Walker A, Walker B, and ABC signature motifs of nucleotidebinding domain (NBD) are highly conserved, but less the Q and H loops; no significant conservatism could be pointed out as to TMD domain within a single ABC protein and  among different proteins. Expression of rice ABCB genes is highly tissuespecific, and divergence has occurred among the expression profiles of different genes. Expression analysis of rice ABCB genes under abiotic stress suggested that most of the genes are responsive to at least 1 type of stress factors.Ka/Ks calculation indicated that purifying selection is essential to the functional maintenance of genes after duplication.

Cite this article

XU Xing, QIU Jie, XU Yang, XU Chenwu* . Molecular  Evolution and Expression Analysis of Subfamily ABCB Transporter Genes in Rice[J]. Chinese Journal OF Rice Science, 2012 , 26(2) : 127 -136 . DOI: 10.3969/j.issn.10017216.2012.02.001

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