The speciesspecific primer for Trichogramma japonicum Ashmead was designed based on its rDNAITS2 sequence with Primer Premier 5.0. With improvement of the conditions of PCR, a 256bp fragment of T. japonicum could be amplified repeatedly with the specificiallydesigned primer. PCR amplifications of Trichogramma samples were carried out with three pairs of diagnostic primers for T. dendrolimi, T. ostriniae, T. chilonis reported previously, and the designed primer for T. japonicum. The results showed that the specific fragment of each Trichogramma species could be amplified with corresponding specific primers, respectively. Furthermore, the results of molecular identification were consistent with those based on morphological identification of male genitalia.
GUO Zhen1, RUAN Changchun1, ZANG Liansheng1,*, ZHANG Fan2,*, JIN Fengyun3
. Design of Specific Primer for Trichogramma japonicum Based on rDNAITS2 and Application of Diagnostic Primers in Identification of Four Trichogramma Species[J]. Chinese Journal OF Rice Science, 2012
, 26(1)
: 123
-126
.
DOI: 10.3969/j.issn.10017216.2012.01.019
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