Genetic analysis for the flag leaf angle was conducted using six generations of P1, P2, F1, B1(F1/P1), B2(F2/P2) and F2derived from the cross between 863B(a maintainer line of japonica rice) and A7444(a germplasm with large flag leaf angle). Genotypes and phenotypes of flag leaf angle were investigated in 863B (P1), A7444 (P2) and 141 B1 plants. An SSR genetic linkage map was constructed and QTLs for flag leaf angle were detected. The genetic map containing 79 information loci was constructed,covering a total distance of 441.6 cM, averaging 5.6 cM between two neighboring loci. Results showed that the trait was controlled by two major genes plus polygene with dominance of major genes. Fifteen markers showed highly significant correlations with flag leaf angle based on single marker regression analysis. Two QTLs， qFLA2 and qFLA8 for flag leaf angle were detected at the intervals of RM300-RM145 and RM6215-RM8265, respectively with WinQTLCart 2.5 and QTL Network 2.0. The positive alleles at the two QTLs were both contributed by A7444.
HU Wende1,2,#, ZHANG Hong1,#, JIANG Jianhua1, WANG Yingying1, SUN Dayun1, WANG Xiaoshuai1, HONG Delin1,*
. Discovery of a Germplasm with Large Flag Leaf Angle and Its Genetic Analysis as well as QTL Mapping in japonica Rice[J]. Chinese Journal OF Rice Science, 2012
, 26(1)
: 34
-42
.
DOI: 10.3969/j.issn.10017216.2012.01.007
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