The expression level of snoRNA transcripts was investigated under PEGstimulated drought stress by Affymetrix 60 k rice genome array. The expression of rice snoRNA transcripts was cultivarspecific under drought stress. A total of 23 snoRNA transcripts from Aihua 5 and only two snoRNA transcripts from Xiangfengzao 119 were upregulated under the osmotic stress. The snoRNA transcripts from two cultivars were box C/D type and presented in five gene clusters on the chromosomes for these snoRNA transcripts. Moreover, an overlap transcript encoding Z274 snoRNA from two cultivars was obtained, but the changed fold of the Z274 snoRNA transcript from Aihua 5 was 1654 fold more than that of Xiangfengzao 119.
MA Tingchen1,2, CHEN Rongjun3, YU Rongrong1, ZENG Hanlai2, ZHANG Duanpin2,*
. Changes in Small Nucleolar RNA Transcripts in Rice Roots under Osmotic Stress by Microarray[J]. Chinese Journal OF Rice Science, 2012
, 26(1)
: 16
-20
.
DOI: 10.3969/j.issn.10017216.2012.01.004
\[1\]吴波， 卢琦. 我国荒漠化基本特点及加快荒漠化地区发展的意义. 中国人口·资源与环境, 2002, 12(1): 99101.
\[2\]Maden B E, Hughes J M. Eukaryotic ribosomal RNA: The recent excitement in the nucleotide modification problem. Chromosoma, 1997， 105(7/8): 391400.
\[3\]Bachellerie J P, Cavaille J, Huttenhofer A. The expanding snoRNA world. Biochimie, 1997, 84: 775790.
\[4\]Samarsky D A, Fournier M J, Singer R H, et al. The snoRNA box C/D motif directs nucleolar targeting and also couples snoRNA synthesis and localization. EMBOJ, 1998, 17: 37473757.
\[5\]KissLászló Z, Henry Y, Kiss T. Sequence and structural elements of methylation guide snoRNAs essential for sitespecific ribose methylation of prerRNA. EMBO J, 1998, 17(3): 797807.
\[6\]Cavaillé J, Nicoloso M, Bachellerie J P. Targeted ribose methylation of RNA in vivo directed by tailored antisense RNA guides. Nature, 1996, 383(6602): 732735.
\[7\]KissLászló Z, Henry Y, Bachellerie J P, et al. Sitespecific ribose methylation of preribosomal RNA: A novel function for small nucleolar RNAs. Cell, 1996, 85(7): 10771088.
\[8\]Ganot P, CaizerguesFerrer M, Kiss T. The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation. Genes Dev, 1997, 11(7): 941956.
\[9\]Jády B E, Kiss T. A small nucleolar guide RNA functions both in 2′Oribose methylation and pseudouridylation of the U5 spliceosomal RNA. EMBO J, 2001, 20(3): 541551.
\[10\]Darzacq X, Jády B E, Verheggen C, et al. Cajal bodyspecific small nuclear RNAs: A novel class of 2′Omethylation and pseudouridylation guide RNAs. EMBO J, 2002, 21(11): 27462756.
\[11\]Gingeras T R. Origin of phenotypes: Genes and transcripts. Genome Res, 2007, 17(6): 682690.
\[12\]Kishore S, Stamm S. The snoRNA HBII52 regulates alternative splicing of the serotonin receptor 2C. Science, 2006, 311 (5758): 230231.
\[13\] 魏敏, 熊建华, 李阳生， 等. 实时PCR定量分析干旱胁迫下水稻糖原合成酶激酶基因表达差异. 中国水稻科学, 2006, 20(6): 567571.
\[14\]Brown J W S, Echeverria M, Qu L H. Plant snoRNAs: Functional evolution and new modes of gene expression. Trend Plant Sci, 2003, 8: 4248.
