A semidwarf and small panicle mutant was obtained from a japonica rice variety Zhonghua 11 through TDNA transformation. The mutant, characterized by semidwarf plant, abnormal small and semienclosed panicle, and poor growth potential was designated as sdsp2(semidwarf and small panicle 2). Genetic analysis indicated that the mutant was controlled by a single recessive gene. A genetic linkage map was constructed using an F2 mapping population derived from a cross between the sdsp2 mutant and an indica variety Longtefu B on chromosome 6. The gene was finally located on a 116kb region between the STS markers RH632 and RH640，where 14 ORFs were predicted based on Rice Genome Annotation．
WANG Qing1,2,3, WANG Dekai2,* , TAO Yuezhi1,2,*
. Genetic Analysis and Gene Mapping of a New SemiDwarf and Small Panicle Mutant in Rice[J]. Chinese Journal OF Rice Science, 2011
, 25(6)
: 677
-680
.
DOI: 10.3969/j.issn.10017216.2011.06.017
\[1\]Khush G. Productivity improvements in rice. Nutri Rev, 2003, 61: S114S116.
\[2\]徐正进, 陈温福, 张龙步, 等. 水稻理想穗型设计的原理与参数. 科学通报, 2005, 50(18): 20372039.
\[3\]郭龙彪, 程式华, 钱前. 水稻基因设计育种的研究进展与展望. 中国水稻科学, 2008, 22(6): 650657.
\[4\]Wang Y, Li J. Molecular basis of plant architecture.Annu Rev Plant Biol, 2008, 59: 253279.
\[5\]Komatsu M, Maekawa M, Shimamoto K, et al. The LAX1 and FRIZZY PANICLE 2 genes determine the inflorescence architecture of rice by controlling rachisbranch and spikelet development. Dev Biol, 2001, 231(2): 364373.
\[6\]Komatsu M, Chujo A, Nagato Y, et al. FRIZZY PANICLE is required to prevent the formation of axillary meristems and to establish floral meristem identity in rice spikelets. Development, 2003, 130(16): 38412850.
\[7\]Li X Y, Qian Q, Fu Z M, et al. Control of tillering in rice. Nature, 2003, 422(6932): 618621.
\[8\]Komatsu K M, Maekawa S, Ujiie Y, et al. LAX and SPA: major regulators of shoot branching in rice. Proc Natl Acad Sci USA, 2003,100(20): 1176511770.
\[9\]Nakagawa M, Shimamoto K, Kyozuka J. Overexpression of RCN1 and RCN2, rice TERMINAL FLOWER 1/CENTRORADIALIS homologs, confers delay of phase transition and altered panicle morphology in rice. Plant J, 2002, 29(6):743750.
\[10\]Moon S, Jung K H, Lee D E, et al. The rice FON1 gene controls vegetative and reproductive development by regulating shoot apical meristem size. Mol Cells, 2006, 21(1): 147152.
\[11\]Chu H, Qian Q, Liang W,et al. The floral organ number4 gene encoding a putative ortholog of Arabidopsis CLAVATA3 regulates apical meristem size in rice. Plant Physiol, 2006,142(3), 10391052.
\[12\]Ikeda K, Ito M, Nagasawa N, et al. Rice ABERRANT PANICLE ORGANIZATION1, encoding an Fbox protein, regulates meristem fate. Plant J, 2007,51(6):10301040.
\[13\]IkedaKawakatsu K, Yasuno N, Oikawa T, et al. Expression level of ABERRANT PANICLE ORGANIZATION1 determines rice inflorescence form through control of cell proliferation in the meristem. Plant Physiol, 2009, 150(2): 736747.
\[14\]Ashikari M, Sakakibara H, Lin S,et al. Cytokinin oxidase regulates rice grain production. Science, 2005,309(5735):741745.
\[15\]Li S B, Qian Q, Fu Z M, et al. Short panicle1 encodes a putative PTR family transporter and determines rice panicle size. Plant J, 2009, 58(4): 592605.
\[16\]Huang X, Qian Q, Liu Z H, et al. Natural variation at the DEP1 locus enhances grain yield in rice. Nat Genet, 2009, 41(4): 494497.
\[17\]Piao R, Jiang W, Ham T H,et al. Mapbased cloning of the ERECT PANICLE 3 gene in rice. Theor Appl Genet, 2009, 119(8): 14971506.
\[18\]Zhu K, Tang D, Yan C,et al. Erect panicle2 encodes a novel protein that regulates panicle erectness in indica rice.Genetics, 2010, 184(2): 343350.
\[19\]Rogers S O, Bendich A J. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol, 1985, 5(2): 6976.
\[20\]Michelmore R W, Paran I, Kesseli R V, et al. Identification of markers linked to diseaseresistance genes by bulked segregation analysis: A rapid method to detect markers in specific genomic regions by using segregation population. Proc Natl Acad Sci USA, 1991, 88(21): 98289832.
\[21\]Panaud O, Chen X, McCouch S R. Development microsatellite markers characterization of simple sequence length polymorphism (SSLPs) in rice (Oryza sativa L.). Mol Gen Genet, 1996, 259(5): 597607.
\[22\]Lander E S, Green P, Abrahamson J, et al. Mapmaker: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1987, 1(2): 174181.
