Using primers designed according to the published sequence of OsCRY1a gene， part of the gene fragment was obtained by PCR and a RNAi expression vector was constructed. The vector was then introduced into rice, leading to the downregulation of the expression level and lossoffunction of the gene. Based on the performance of the transgenic plants, the functions of OsCRY1a was analyzed and deduced. The results indicated that suppressing the expression of OsCRY1a retarded flowering for 16 d with the plant height and grain length significantly increased while other important agronomic traits remained unchanged apparently.
LI Yu1,2，ZHUANG Weijian1,3,*，WANG Naiyuan2，HONG Guoqin1，DAI Fei3
. Effects of Suppressing OsCRY1a Gene Expression on Rice Agronomic Traits[J]. Chinese Journal OF Rice Science, 2011
, 25(6)
: 575
-579
.
DOI: 10.3969/j.issn.10017216.2011.06.002
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