Research Papers

Primary Function Analysis of a  Pyruvate Decarboxylase Gene, OsPDC3, in Rice

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  • National Center for Gene Research/Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, 
    Chinese Academy of Sciences, Shanghai  200233, China

Received date: 2011-05-04

  Revised date: 2011-05-25

  Online published: 2011-11-10

Abstract

There are high aerobic fermentation rates in plant pollen. As  a key enzyme of ethanolic fermentation pathway, pyruvate decarboxylase (PDC) is involved in  energy production and biosynthesis in pollen,  playing  an important role in the pollen germination process.OsPDC3  gene function was investigated by the reverse genetics methods. The gene is  a single exon gene encoding a protein that shares      77%-82%   sequence identity  with other rice PDC proteins. Expression pattern analysis showed  that the OsPDC3  specifically expressed in pollen, and  the pollen specific expression activity of the promoter of  OsPDC3 was confirmed by GUS staining. Overexpression of OsPDC3 in transgenic plants improved  the    activity of PDC in leaves, indicating functional enzyme activity of OsPDC  in vivo.  Antisense suppression downregulated  the expression of OsPDC3 in pollen, but the pollen germination rate in vitro  remained  unchangable, which might attribute to  the redundancy among the PDC genes.

Cite this article

CHEN Bo, HAN Bin* . Primary Function Analysis of a  Pyruvate Decarboxylase Gene, OsPDC3, in Rice[J]. Chinese Journal OF Rice Science, 2011 , 25(6) : 567 -574 . DOI: 10.3969/j.issn.10017216.2011.06.001

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