Secreted proteins during spore germination of Magnaporthe oryzaewere identified. Ungerminated spores, germinated spores and the mixture of germinated spores with germination liquid of M. oryzae (200550, 200581) were inoculated on rice seedlings, respectively. The disease indices were 1113, 3506, 4901 and 4901, 84, 627, indicating that germination liquid played a driving role in infection process. SDSPAGE amplification revealed that secreted proteins were contained. According to the results of mass spectrometer, and database query, eight genes were involved, including MGG_01305, MGG_13188, MGG_12454, etc. The results indicated that these proteins played key roles and important function during M. oryzae infection.
LIU Yong-Feng, CHEN Zhi-Yi, YU Wen-Yuan, YU Jun-Jie, HU Jian-Kun, LIU You-Zhou, NIE Ya-Feng
. Primary Study on Effect of Secreted Proteins of Magnaporthe oryzae During Infection[J]. Chinese Journal OF Rice Science, 2011
, 25(4)
: 452
-454
.
DOI: 10.3969/j.issn.1001-7216.2011.04.018
[1]陆凡, 王法明, 郑小波, 等. 江苏省稻瘟病菌小种的演变与水稻品种关系的研究. 南京农业大学学报, 1999, 22(4): 31-34.
[2]陆凡, 郑小波, 陈志谊, 等. 江苏省稻瘟病菌的毒性多样性及水稻品种的抗病性. 生物多样性, 2001, 9(3): 201-206.
[3]林菁菁, 李进斌, 刘林, 等. 云南元阳哈尼梯田稻瘟病菌遗传多样性分析. 植物病理学报, 2009, 39(1): 43-51.
[4]史阿宝, 王法明, 陈毓苓, 等. 稻瘟病菌孢子萌发液中诱导侵染活性物质初探. 植物保护学报, 1997, 34(3): 221-224.
[5]Liu Y F, Chen Z Y, Hu M, et al. Distribution of Magnaporthe oryzae populations and virulence of predominant races in Jiangsu Province. Rice Sci, 2004, 11(5/6): 324-330.
[6]Zhou E X, Jia Y L, Singh P, et al. Instability of the Magnaporthe oryzae avirulence gene AVR-Pita alters virulence. Fungal Genet Biol, 2007, 44: 1024-1034.
[7]Chen X W, Shang J J, Chen D X, et al. A B-lectin receptor kinase gene conferring rice blast resistance. Plant J, 2006, 46: 794-804.
[8]林福呈, 李德葆. 稻瘟病菌分生孢子发芽和附着胞形成的影响因素研究. 中国水稻科学, 2001, 15(4): 291-297.
[9]张传清, 周明国. 超氧自由基在三环唑防治稻瘟病中的作用. 植物病理学报, 2004, 34(5): 437-411.
[10]丁克坚, 檀根甲, 高智谋, 等. 影响水稻稻瘟病菌侵染过程的生态因子研究. 应用生态学报, 2002, 13: 698-700.
[11]王艳丽, 张正光, 陆凡, 等. 稻瘟病菌无性重组体若干生物学性状的研究. 南京农业大学学报, 2003, 26: 33-37.
[12]Rubio M B, Cardoza R E, Hermosa R, et al. Cloning and characterization of the Thcut1 gene encoding a cutinase of Trichoderma harzianum T34. Curr Genet, 2008, 54(6): 301-312.
[13]Sweigard J A, Chumley F G, Valent B. Cloning and analysis of CUT1, a cutinase gene from Magnaporthe grisea. Mol Gen Genet, 1992, 232: 174-182.
[14]汪家政, 范明. 蛋白质技术手册. 北京: 科学出版社, 2002: 77-99.
[15]盛泉虎, 解涛, 丁达夫. 串联质谱数据的从头解析与蛋白质的数据库搜索鉴定. 生物物理和生物化学学报, 2000, 32: 595-600.
[16]Lu J P, Feng X X, Liu X H, et al. Mnh6, a nonhistone protein, is required for fungal development and pathogenicity of Magnaporthe grisea. Fungal Genet Biol, 2007, 44(9): 819-829.
[17]Skamnioti P, Gurr S J. Magnaporthe grisea Cutinase2 mediates appressorium differentiation and host penetration and is required for full virulence. Plant Cell, 2007, 19: 2674-2689.
[18]Yi M, Park J H, Ahn J H, et al. MoSNF1 regulates sporulation and pathogenicity in the rice blast fungus Magnaporthe oryzae. Fungal Genet Biol, 2008, 45(8): 1172-1181.
[19]Zhou J, Zheng X Z, Lan L, et al. Biochemical and molecular characterization of a putative endoglucanase in Magnaporthe grisea. Curr Genet, 2008, 53(4): 217-224.