Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2009 , Vol. 23 >Issue 6: 589 - 594

DOI: https://doi.org/10.3969/j.issn.1001-7216.2009.06.05

研究报告

Detection of QTL for Alkali Tolerance at the Germination Stage in japonica Rice

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  • 1Institute of Crop Science, Chinese Academy of Agricultural Sciences/The National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI/Key Laboratory of Crop Germplasm Resources and Biotechnology, Ministry of Agriculture, Beijing 100081, China； 2Institute of Rubber Research, Chinese Academy of Tropical Agricultural Sciences/State Engineering and Technology Research Center for Key Tropical Crops/State Key Laboratory Breeding Base of Cultivation & Physiology for Tropical Crops,Hainan Provincial Key Laboratory for Tropical Crops Physiology, Danzhou 571737, China; 3National Academy of Agricultural Sciences, RDA, Suwon 441707, Korea； 4Institute of Rice Research, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China； 5Horticultural Department, Sichuan Agricultural University, Ya′an 625014, China; *Corresponding authors, E-mail: lzhan58@yahoo.com.cn, jlgzszsy12@126.com

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2009-11-10

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Abstract

The quantitative trait loci (QTLs) for germination capacity and relative alkali damage rate for germination capacity in rice under alkali stress (0.15% Na2CO3 solution) were identified by using an F2:3 population, which included 200 individuals and lines derived from a cross between two japonica cultivars Gaochan 106 and Changbai 9 with microsatellite markers. The germination capacity and its relative alkali damage rate showed a continuous and near normal distribution in F3 lines. Seven QTLs associated with the germination capacity under alkali stress were detected, which explained 4.05% to 12.61% of the observed phenotypic variances. qGC6 and qGC9 were located in RM225-RM204 and RM219-RM3700, respectively, which accounted for 12.61% and 10.85% of the observed phenotypic variation, respectively. Six QTLs correlated with relative alkali damage rate for germination capacity under alkali stress were detected. qRGC2, qRGC61, qRGC9, and qRGC12 were major QTLs located in RM29-RM221, RM225-RM204, RM219-RM3700, and RM260-RM3226, respectively, which explained 28.07%, 15.35%, 15.61% and 18.91% of the observed phenotypic variation, respectively. Given the long distances between the markers further research should be conducted. Most of the alleles were partially dominant or overdominant.

Key words： rice; alkali tolerance; germination capacity; relative alkali damage rate; microsatellite marker; quantitative trait locus

Cite this article

QI Dong-ling,LEE Jung-ro,YANG Chun-gang,LEE Myung-chul,CAO Gui-lan,ZHANG Jun-guo,ZHOU Qing-yang,SUH Seok-cheol,ZHANG San-yuan,HAN Long-zhi . Detection of QTL for Alkali Tolerance at the Germination Stage in japonica Rice[J]. Chinese Journal OF Rice Science, 2009 , 23(6) : 589 -594 . DOI: 10.3969/j.issn.1001-7216.2009.06.05

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