Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2009 , Vol. 23 >Issue 5: 463 - 469

DOI: https://doi.org/10.3969/j.issn.10017216.2009.05.03

研究报告

Identification of a Specific Avirulence Gene from a Chinese Race C8 of Xanthomonas oryzae pv. oryzae

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  • 1 College of Plant Protection/Key Laboratory for Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China; 2 College of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China; # These authors contributed equally to this paper; *Corresponding author, E-mail: gyouchen@njau.edu.cn

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2009-09-10

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Abstract

The interaction between Xanthomonas oryzae pv. oryzae (Xoo) and rice follows geneforgene model. A 6.0 kb particular fragment was identified from a Chinese race C8 strain of Xoo, through screening its genomic library by Southern blot with a highly conserved EcoRⅠBamHⅠ fragment of the avrBs3/pthA family as probe. This specific DNA contained an avirulence gene, namely avr/pthC8a, which transmitted the Philippine strain PXO99A from compatible into incompatible interactions with rice Asominori carrying R gene Xa17, suggesting that avr/pthC8a is possibly corresponding for Xa17 specificity. Sequence analysis showed that avr/pthC8a, 3819 bp in size, is a avrBs3/pthA family member, and the repeat number of the 102 bp repeat unit is 20.5. The structure of the gene is almost as same as those of the avrBs3/pthA family genes at 5′ and 3′terminals. The putative protein has a lucine zipper, three nucleotide location signals and an acid transcriptional activation domain at Cterminal. The novel is that the BamHⅠ site at 3′terminal of the gene is mutagenesis from GGATCC into AGATCC, which has not been reported so far. Thus, identification of avr/pthC8a of Chinese race C8 provides a fundamental understanding of virulence differentiation among different races of Xoo and coevolution of avrBs3/pthA genes with rice.

Key words： rice; Xathomonas oryzae pv. oryzae; avirulence gene; gene function

Cite this article

JI Zhi-yuan,YANG Juan,WANG Yin-peng,ZHOU Dan,LI Yu-rong,ZOU Li-fang,CHEN Gong-you . Identification of a Specific Avirulence Gene from a Chinese Race C8 of Xanthomonas oryzae pv. oryzae
[J]. Chinese Journal OF Rice Science, 2009 , 23(5) : 463 -469 . DOI: 10.3969/j.issn.10017216.2009.05.03

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