Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2011 , Vol. 25 >Issue 3: 331 - 334

DOI: https://doi.org/10.3969/j.issn.1001-7216.2011.03.016

研究简报

Breeding of Rice Lines with Bacterial Blight Resistance Gene Xa23 by Using MarkerAssisted Selection

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  • 1 College of Chemistry and Life Sciences, Zhejiang Normal University, Hangzhou 321004, China; 2 The Institute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; * Corresponding author, E-mail: zwsjsxz @zaas.org

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2011-05-10

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Abstract

The rice bacterial blight resistance gene Xa23, which is carried by the donor parent 18113, was introduced into the intersubspecific restorer line H705 and good grain quality restorer line H706 by crossing and molecular markerassisted selection(MAS). Seventyone and 52 homozygous lines carrying Xa23 were obtained from the 18113/H705 F3 and 18113/H706 F3 lines, respectively by PCR with EST marker C189. The bacterial blight resistance of these lines was investigated with the pathotype Ⅳ representative strain Zhe 173 by clipping method. Sixtyone and 44 lines showed the bacterial blight resistance, with the MAS accuracies of 85.92% and 84.61%, respectively. The C189 was one of the effective MAS markers for testing Xa23 gene. In addition, further research is needed to elucidate the mechanisms of higher false positive rate than theoretical exchange rate.

Key words： rice; bacterial blight; markerassisted selection; resistance gene; breeding

Cite this article

FAN Hong-huan,WANG Lin-you,ZHANG Li-xia,YU Xin,WANG Xi,JIN Qing-sheng,WANG Jian-jun . Breeding of Rice Lines with Bacterial Blight Resistance Gene Xa23 by Using MarkerAssisted Selection[J]. Chinese Journal OF Rice Science, 2011 , 25(3) : 331 -334 . DOI: 10.3969/j.issn.1001-7216.2011.03.016

References

[1]Mew T W. Curent status and future prospects of research on bacterial blight of rice. Annu Rev Phytopathol, 1987, 25: 359-382.
[2]Yoshimura S, Yamanouchi U, Katayose Y, et al. Expression of Xa1, a bacterial blight resistance gene in rice, is induced by bacterial inoculation. Proc Natl Acad Sci USA, 1998, 95(4): 1663-1668.
[3]Lyer A S, McCouch S R. The rice bacterial blight resistance gene xa5 encodes a novel form of disease resistance. Mol Plant-Microbe Interact, 2004, 17(12): 1348-1354.
[4]Chu Z H, Yuan M, Yao J L, et al. Promoter mutations of an essential gene for pollen development result in disease resistance in rice. Genes Dev, 2006, 20(10): 1250-1255.
[5]Song W Y, Wang G L, Chert L L, et al. A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Science, 1995, 270(5243): 1804-1806.
[6]Sun X L, Cao Y L, Yang Z F, et al. Xa26, a gene conferring resistance to Xanthomonas oryzae pv. oryzae in rice, encodes an LRR receptor kinase-like protein. Plant J, 2004, 37(4): 517-527.
[7]Gu K Y, Yang B, Tian D S, et al. R gene expression induced by a type-Ⅲ effector triggers disease resistance in rice. Nature, 2005, 435(7045): 1122-1125.
[8]刘丙新, 季芝娟, 石建尧, 等. 水稻白叶枯病抗性基因的发掘及在育种中的应用. 中国稻米, 2010, 16(2): 10-15.
[9]曾列先, 成太辉, 朱小源, 等. 广东首个抗白叶枯病强毒菌系V型菌水稻品种白香占的选育. 广东农业科学, 2009(5): 19, 28.
[10]易懋升, 丁效华, 张泽民, 等. 水稻抗白叶枯病恢复系的分子育种. 华南农业大学学报, 2006, 27(2): 1-4.
[11]曹立勇, 庄杰云, 占小登, 等. 抗白叶枯病杂交水稻的分子标记辅助育种. 中国水稻科学, 2003, 17(2): 184-186.
[12]邓其明, 王世全, 郑爱萍, 等. 利用分子标记辅助育种技术选育高抗白叶枯病恢复系. 中国水稻科学, 2006, 20(2): 153-158.
[13]曾列先, 黄少华, 伍尚忠. IRBB21(Xa21)对广东稻白叶枯病菌5个小种的抗性反应. 植物保护学报, 2002, 29(2): 97-100.
[14]曾列先, 朱小源, 杨建源, 等. 广东水稻白叶枯病菌新致病型的发现及致病性鉴定. 广东农业科学, 2005, 29(2): 58-59.
[15]姬广海, 张世光, 钱君. 云南水稻白叶枯病菌生理小种初析. 植物保护, 2003, 29(1): 19-21.
[16]章琦, 赵炳宇, 赵开军, 等. 普通野生稻抗水稻白叶枯病(Xanthomonas oryzae pv. oryzae)新基因Xa23(t)的鉴定和分子标记定位. 作物学报, 2000, 26(5): 536-542.
[17]王麒. 分子标记辅助选择及其在水稻育种中应用与展望. 黑龙江农业科学, 2009(1): 140-143.
[18]王建军, 朱旭东, 王林友, 等. 水稻类病变突变体lrd40的抗性与细胞学分析. 中国水稻科学, 2005, 19(2): 111-116.
[19]王春连, 戚华雄, 潘海军, 等. 水稻抗白叶枯病基因Xa23的EST标记及其在分子育种上的利用. 中国农业科学, 2005, 38(10): 1996-2001.
[20]Zhang Q F, Saghai Maroof M A, Lu T Y, et al. Genetic diversity and differential of indica and japonica rice detected by RFLP analysis. Theor Appl Genet, 1992, 83(4): 495-499.
[21]吕瑞玲, 吴小凤, 刘敏超. 分子标记技术及在水稻遗传研究中的应用. 中国农学通报, 2009, 25(4): 65-73.
[22]王兴春, 杨长登, 李西明, 等. 分子标记辅助选择与花药培养相结合快速聚合水稻白叶枯病抗性基因. 中国水稻科学, 2004, 18(1): 7-10.
[23]黄廷友, 李仕贵, 王玉平, 等. 蜀恢527 抗水稻白叶枯病改良系的遗传背景和农艺性状分析. 作物学报, 2004, 30(6): 563-567.
[24]李进波, 王春连, 戚华雄, 等. 分子标记辅助选择Xa23基因培育杂交稻抗白叶枯病恢复系. 作物学报, 2006, 32(10): 1423-1429.
[25]潘海军, 王春连, 赵开军, 等. 水稻抗白叶枯病基因Xa23的PCR分子标记定位及辅助选择. 作物学报, 2003, 29(4): 501-507.
[26]Chen H, Wang S, Zhang Q. A new gene for bacterial blight resistance in rice located on chromosome 12 identified from Minghui 63, an elite restorer line. Phytopathology, 2002, 92(7): 750-754.
[27]Ogawa T, Yamamoto T, Khush G S, et al. Breeding of near-isogenic lines of rice with single genes for resistance to bacterial blight pathogen (Xanthomonas campestris pv. oryzae). Jpn J Breeding, 1991, 41(33): 523-529.
[28]Xiang Y, Cao Y, Xu C, et al. Xa3, conferring resistance for rice bacterial blight and encoding a receptor kinase-like protein, is the same as Xa26.Theor Appl Genet, 2006, 113(7): 1347-1355.
[29]Banerjee D, Zhang X, Bent A F. The leucine- rich repeat domain can determine effective interaction between RPS2 and other host factors in Arabidopsis RPS2-mediated disease resistance. Genetics, 2001, 158(1): 439-450.
[30]Cao Y, Ding X, Cai M, et al. The expression pattern of a rice disease resistance gene Xa3/Xa26 is differentially regulated by the genetic backgrounds and developmental stages that influence its function. Genetics, 2007, 177(1): 523-533.
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