Hua 201S is an elite photo-thermo-sensitive genic male sterile(PTGMS) line, which was used for developing two-line hybrid rice. A bacterial blight resistant breeding line ‘Huahui 20’ containing Xa21 and Xa7 genes as donor parent was crossed with Hua 201S. F1 was backcrossed to Hua 201S for three times. The BC3F1 progenies were advanced to BC3F3 by selfing. The primers PTA248 for Xa21 and STSP3 (genetic distance to Xa7 is 0.9 cM) for Xa7 were used for molecular marker-assisted selection in each progeny. The fertility alterations, main agronomic traits and growth durations of the four selected lines YR7009(Xa21), YR7014(Xa21), YR7016(Xa7) and YR7023(Xa7/Xa21) were similar to the recipient parent Hua 201S. YR7009(Xa21) and YR7014(Xa21) were resistant against four Xanthomonas oryzae pv. oryzae（Xoo） strains, PXO61,PXO99, ZHE173 and GD1358, but not resistant against two Chinese strains, FuJ and YN24. YR7016(Xa7) and YR7023(Xa7/Xa21) were resistant against all the six Xoo strains tested. It showed that Xa21 and Xa7 were introgressed into the PTGMS line Hua 201S, improving the resistance against rice bacterial blight.
LAN Yan-rong#,WANG Jun-yi#,WANG Yi,MOU Tong-min*
. Improvement of Rice Bacterial Blight Resistance of Hua 201S, an Elite Photo-Thermo- Sensitive Genic Male Sterile Line, by Molecular Marker-Assisted Selection[J]. Chinese Journal OF Rice Science, 2011
, 25(2)
: 169
-174
.
DOI: 10.3969/j.issn.1001-7216.2011.02.008
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