<FONT face="Verdana">Identification and Mapping of QTLs for Cold Tolerance at the Seedling Stage in Common Wild Rice(<EM>Oryza rufipogon</EM>) </FONT>

ZHENG Jia-xing,MA Zeng-feng,SONG Jian-dong,LIU Chi,LI Yong-tao,HUANG Da-hui,WEI Shao-li,ZHANG Yue-xiong,MI Ke,HUANG Jin-yan,CHEN Miao,MENG Jiao-rong,LI Rong-bai,CHEN Bao-shan

doi:10.3969/j.issn.1001-7216.2011.01.008

Chinese Journal OF Rice Science >

2011 , Vol. 25 >Issue 1: 52 - 58

DOI: https://doi.org/10.3969/j.issn.1001-7216.2011.01.008

研究报告

Identification and Mapping of QTLs for Cold Tolerance at the Seedling Stage in Common Wild Rice(Oryza rufipogon)

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¹Guangxi Crop Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; ²College of Agriculture/College of Life Science and Technology/Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Guangxi University, Nanning 530005, China; ³Institute of Rice Research, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; ⁴The 43rd Middle School of Nanning, Nanning 530200, China; *Corresponding authors, E-mail: lirongbai@126.com; chenyaoj@gxu.edu.cn)

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2011-01-10

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Abstract

Two core accessions of common wild rice (DP15 and DP30) as donor parents and the cultivar 9311 as recipient parent were used to construct chromosome segment substitution lines for identification of QTLs for cold tolerance at the seedling stage and QTL effect analysis. A QTL mapping population from the most coldtolerant substitution line was constructed to map majoreffect QTLs with SSR markers. The two donor lines, DP15 and DP30, showed obvious difference in the number, location and effect of QTLs for cold tolerance. In the 230 BC4F2 substitution lines in which substituted chromosome segments covered the whole genome of the two donors, a total of 19 cold tolerance QTLs were detected, which distributed throughout all the 12 chromosomes, and clustered on chromosome 3 and chromosome 8. These QTLs were separated each other in different chromosome substitution lines. The substitution line with the smallest effect QTL for cold tolerance and the line with the largest effect QTL showed 8% and 74% of survival rate after cold treatment, respectively. A maineffect QTL, qSCT31, was mapped between SSR markers RM15031 and RM3400, near the centromere on the long arm of chromosome 3 with a distance of 18 cM to the closest linkage markers, RM15040 and RM1164.

Key words： cold tolerance; quantitative trait locus; Oryza rufipogon; chromosome segment substitution line; gene mapping

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ZHENG Jia-xing,MA Zeng-feng,SONG Jian-dong,LIU Chi,LI Yong-tao,HUANG Da-hui,WEI Shao-li,ZHANG Yue-xiong,MI Ke,HUANG Jin-yan,CHEN Miao,MENG Jiao-rong,LI Rong-bai,CHEN Bao-shan . Identification and Mapping of QTLs for Cold Tolerance at the Seedling Stage in Common Wild Rice(Oryza rufipogon) [J]. Chinese Journal OF Rice Science, 2011 , 25(1) : 52 -58 . DOI: 10.3969/j.issn.1001-7216.2011.01.008

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