<FONT face="Verdana">Construction of Molecular Fingerprinting Database and Dual PCR Assay for Two Indica Rice Hybrids and Two Japonica Rice Varieties by Using SSR Markers<BR></FONT>

CHEN Shu-lin,WANG Hai-yan,DING Zhen-qian,WANG Xiu-e,CHEN Zhong-ming

doi:10.3969/j.issn.1001-7216.2011.01.003

2011 , Vol. 25 >Issue 1: 19 - 24

DOI: https://doi.org/10.3969/j.issn.1001-7216.2011.01.003

研究报告

Construction of Molecular Fingerprinting Database and Dual PCR Assay for Two Indica Rice Hybrids and Two Japonica Rice Varieties by Using SSR Markers

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¹State Key Laboratory of Crop Genetics and Germplasm Enhancement/Institute of Cytogenetics, Nanjing Agricultural University, Nanjing 210095, China; ²Jiangsu Mingtian Seeds Science and Technology Co. Ltd/Jiangsu Crop Seeds Engineering Research Center, Nanjing 210014, China; *Corresponding authors, E-mail: hywang@njau.edu.cn; zhongmingchen136@sina.com

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2011-01-10

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Abstract

A total of 460 SSR primers were used for amplification of five indica hybrid rice combinations and their parents, and 16 japonica rice varieties (or elite lines). By using the identified codominant specific primers, molecular fingerprinting databases for Guodao 6, Liangyoupeijiu,Yandao 8 and Xudao 4 were constructed. A total of 5, 3, 1 and 2 SSR primers were identified to be specific for Guodao 6, Liangyoupeijiu, Yandao 8 and Xudao 4, respectively. These fingerprints can be used to examine the seed purity of these rice varieties. In addition, to develop dual PCR assay in examination of seed purity and authenticity, SSR primers which could stably amplify clear bands and have good specificity were selected and used in multiplex popymerase chain reaction amplification.

Key words： hybrid rice; seed purity; seed authenticity; fingerprinting; dual polymerase chain reaction assay; seed identification

Cite this article

CHEN Shu-lin,WANG Hai-yan,DING Zhen-qian,WANG Xiu-e,CHEN Zhong-ming . Construction of Molecular Fingerprinting Database and Dual PCR Assay for Two Indica Rice Hybrids and Two Japonica Rice Varieties by Using SSR Markers
[J]. Chinese Journal OF Rice Science, 2011 , 25(1) : 19 -24 . DOI: 10.3969/j.issn.1001-7216.2011.01.003

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