Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2011 , Vol. 25 >Issue 1: 25 - 30

DOI: https://doi.org/10.3969/j.issn.1001-7216.2011.01.004

研究报告

Development of STS Marker Linked to Rice Blast Resistance Gene Pi9 in Marker Assisted Selection Breeding

Expand
  • Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China; *Corresponding author, E-mail: qihx@hbaas.com

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2011-01-10

Fold

Abstract

Indica rice line 751127 carrying broadspectrum rice blast resistance gene Pi9 was used as resistance gene donor for genetic improvement of Yangdao 6 and R6547. By alignment of genomic sequences from the Pi9 locus of 751127, Yangdao 6 and Nipponbare, codominant STS (sequencetagged site) marker PB91, which linked to the Pi9 gene, was developed for molecular markerassisted selection (MAS). Eight backcross lines carrying Pi9 were obtained, among which four lines contained the genetic background of Yangdao 6 and the other four lines had the R6547 background. Under natural disease infection conditions in Enshi County and Yichang County of Hubei Province, China, the Pi9carrying lines showed higher blast disease resistance than Yangdao 6 and R6547 in various degrees. The hybrids originated from 08C893, an R6547derived rice line, showed higher blast disease resistance as compared to the control Yangliangyou 6. The results suggest PB91 is an effective codominant marker for MAS of Pi9 which is a valuable resistance gene for the control of rice blast disease in Hubei area.

Key words： blast resistance; molecular markerassisted selection; sequencetagged site; resistance gene; breading; rice

Cite this article

YIN De-suo,XIA Ming-yuan,LI Jin-bo,WAN Bing-liang,ZHA Zhong-ping,DU Xue-shu,QI Hua-xiong* . Development of STS Marker Linked to Rice Blast Resistance Gene Pi9 in Marker Assisted Selection Breeding
[J]. Chinese Journal OF Rice Science, 2011 , 25(1) : 25 -30 . DOI: 10.3969/j.issn.1001-7216.2011.01.004

References

[1]Baker B, Zambryski P, Staskawicz B, et al. Signaling in plant-microbe interactions. Science, 1997, 276(5313): 726-733.
[2]董继新, 董海涛, 李德葆. 水稻抗瘟性研究进展. 农业生物技术学报, 2000, 8(1): 99-120.
[3]杨勤忠, 林菲, 冯淑杰, 等. 水稻稻瘟病抗性基因的分子定位及克隆研究进展. 中国农业科学, 2009, 42(5): 1601-1615.
[4]吴俊, 刘雄伦, 戴良英, 等. 水稻广谱抗稻瘟病基因研究进展. 生命科学, 2007, 19(2): 233-238.
[5]Hittalmani S, Parco A, Mew T W, et al. Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice. Theor Appl Genet, 2000, 100(7):1121-1128.
[6]Narayanan N N, Baisakh N, Oliva N P, et al. Molecular breeding: Marker-assisted selection combined with biolistic transformation for blast and bacterial blight resistance in indica rice (cv. CO39). Mol Breed, 14(1): 61-71.
[7]陈学伟, 李世贵, 黎汉云, 等. 水稻抗稻瘟病基因Pi-d(t)、Pi-b、 Pi-ta2的聚合及分子标记选择. 生物工程学报, 2004, 20(5): 708-714.
[8]陈志伟, 郑燕, 吴为人, 等. 抗稻瘟病基因Pi-2(t)紧密连锁的SSR标记的筛选与应用. 分子植物育种, 2004, 2(3): 321-325.
[9]柳武革, 王丰, 金素娟, 等. 利用分子标记辅助选择聚合Pi-1和Pi-2基因改良两系不育系的稻瘟病抗性. 作物学报, 2008, 34(7): 1128-1136.
[10]陈红旗, 陈宗祥, 倪深, 等. 利用分子标记技术聚合3个稻瘟病基因改良金23B的稻瘟病抗性. 中国水稻科学, 2008, 22(1): 23-27.
[11]倪大虎, 易成新, 李莉, 等. 利用分子标记辅助选择聚合水稻基因Xa21和Pi9(t). 分子植物育种, 2005, 3(3): 329-334.
[12]陈健民, 付志英, 王锋, 等. 分子标记辅助培育双抗稻瘟病和白叶枯病杂交稻恢复系. 分子植物育种, 2009, 7(3): 465-470.
[13]Liu G F, Lu G, Zeng L R, et al. Two broad-spectrum blast resistance genes, Pi9(t) and Pi2(t), are physically linked on rice chromosome 6. Mol Genet Genomics, 2002, 267(4): 472-480.
[14]Qu S H, Liu G F, Wang G L, et al. The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice. Genetics, 2006, 172(3): 1901-1914.
[15]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acid Res, 1980, 8(19): 4321-4325.
[16]李进波, 夏明元, 戚华雄, 等. 水稻抗褐飞虱基因Bph14和Bph15的分子标记辅助选择. 中国农业科学, 2006, 39(10): 2132-2137.
Options
Outlines

/

〈 〉

Tel: 0571-63370278　E-mail: cjrs@263.net
Supported by Beijing Magtech Co., Ltd.