综述与专论

Recent Advances in Proteomic Studies on Rice Pathogen Interactions

Expand
  • Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; *Corresponding author, E-mail: jpchen2001@yahoo.com.cn

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2010-11-10

Abstract

The molecular mechanisms of plantpathogen interactions have been a longtime hotspot of plant pathology. Recently, functional genomic strategies, including proteomics and transcriptomics, have been widely used in defining gene and protein function and expression profiles. Proteomics, a complementary research content of transcriptomics, has been a powerful tool to systematically analyze the cellular protein expression profiles and posttranslational modifications under pathogen invasion. Rice, a monocotyledonous plant for which the entire genome was completely sequenced, has been adopted as an ideal model for studying the molecular mechanisms of plantpathogen interactions. In this review, the main research techniques of proteomics, recent advances in proteomic analyses of rice under bacteria, fungi and viruses invasion were highlighted, and an overview on phosphoproteomics analyses of ricepathogen interactions was presented.

Cite this article

YU Chu-lang,YANG Yong,WANG Xu-ming,YAN Cheng-qi,CHEN Jian-ping* . Recent Advances in Proteomic Studies on Rice Pathogen Interactions [J]. Chinese Journal OF Rice Science, 2010 , 24(6) : 647 -651 . DOI: 10.3969/j.issn.1001-7216.2010.06.014

References

[1]Staskawicz B J. Genetics of plant-pathogen interactions specifying plant disease resistance. Plant Physiol, 2001, 125(1): 73-76.
[2]Gygi S P, Rochon Y, Franza B R, et al. Correlation between protein and mRNA abundance in yeast. Mol Cell Biol, 1999, 19(3): 1720-1730.
[3]Lee S W, Han S W, Bartley L E, et al. From the academy: Colloquium review. Unique characteristics of Xanthomonas oryzae pv. oryzae AvrXa21 and implications for plant innate immunity. Proc Natl Acad Sci USA, 2006, 103(49): 18395-18400.
[4]Ebbole D J. Magnaporthe as a model for understanding host-pathogen interactions. Annu Rev Phytopathol, 2007, 45: 437-456.
[5]O’Farrell P H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem, 1975, 250(10): 4007-4021.
[6]Asirvatham V S, Watson B S, Sumner L W. Analytical and biological variances associated with proteomic studies of Medicago truncatula by two-dimensional polyacrylamide gel electrophoresis. Proteomics, 2002, 2(8): 960-968.
[7]Alban A, David S O, Bjorkesten L, et al. A novel experimental design for comparative two-dimensional gel analysis:two-dimensional difference gel electrophoresis incorporating a pooled internal standard. Proteomics, 2003, 3(1): 36-44.
[8]Lilley K S, Friedman D B. Difference gel electrophoresis DIGE. Drug Discov Today: Technol, 2006, 3(3): 347-353.
[9]Baggerman G, Vierstraete E, De Loof A, et al. Gel-based versus gel-free proteomics: A review. Comb Chem High Throughput Screen, 2005, 8(8): 669-677.
[10]Molloy M P, Witzmann F A. Proteomics: Technologies and applications. Brief Funct Genomic Proteomics, 2002, 1(1): 23-29.
[11]Majeran W, Zybailov B, Ytterberg A J, et al. Consequences of C4 differentiation for chloroplast membrane proteomes in maize mesophyll and bundle sheath cells. Mol Cell Proteomics, 2008, 7(9): 1609-1638.
[12]孙言伟, 姜颖, 贺福初. 差异蛋白质组学的研究进展. 生命科学, 2005, 17(2): 137-140.
[13]陈功友, 邹丽芳, 王邢平, 等. 水稻白叶枯病菌致病性分子遗传学基础. 中国农业科学, 2004, 37(9): 1301-1307.
[14]Mahmood T, Jan A, Kakishima M, et al. Proteomic analysis of bacterial-blight defense-responsive proteins in rice leaf blades. Proteomics, 2006, 6(22): 6053-6065.
[15]Yu C L, Yan S P, Wang C C, et al. Pathogenesis-related proteins in somatic hybrid rice induced by bacterial blight. Phytochemistry, 2008, 69(10): 1989-1996.
[16]Chen F, Yuan Y X, Li Q, et al. Proteomic analysis of rice plasma membrane reveals proteins involved in early defense response to bacterial blight. Proteomics, 2007, 7(9): 1529-1539.
[17]陈芳育, 黄青云, 张红心, 等. 水稻品种“佳辐占”应答细菌性条斑病病原菌侵染的蛋白质组学分析. 作物学报, 2007, 33(7): 1051-1058.
[18]Kim S T, Cho K S, Yu S, et al. Proteomic analysis of differentially expressed proteins induced by rice blast fungus and elicitor in suspension-cultured rice cells. Proteomics, 2003, 3(12): 2368-2378.
[19]Kim S T, Kim S G, Hwang D H, et al. Proteomic analysis of pathogen-responsive proteins from rice leaves induced by rice blast fungus, Magnaporthe grisea. Proteomics, 2004, 4(11): 3569-3578.
[20]Kim S T, Kang Y H, Wang Y, et al. Secretome analysis of differentially induced proteins in rice suspension-cultured cells triggered by rice blast fungus and elicitor. Proteomics, 2009, 9(5): 1302-1313.
[21]Ventelon-Debout M, Delalande F, Brizard J P, et al. Proteome analysis of cultivar-specific degradation of Oryza sativa indica and O. sativa japonica cellular suspensions undergoing rice yellow mottle virus infection. Proteomics, 2004, 4(1): 216-225.
[22]Brizard J P, Carapito C, Delalande F, et al. Proteome analysis of plant-virus interactome: Comprehensive data for virus multiplication inside their hosts. Mol Cell Proteomics, 2006, 5(12): 2279-2297.
[23]Pawson T, Scott J D. Protein phosphorylation in signaling: 50 years and counting. Trends Biochem Sci, 2005, 30(6): 286-290.
[24]Hubbard M J, Cohen P. On target with a new mechanism for the regulation of protein phosphorylation. Trends Biochem Sci, 1993, 18(5): 172-177.
[25]Kersten B, Agrawal G K, Iwahashi H, et al. Plant phosphoproteomics: A long road ahead. Proteomics, 2006, 6(20): 5517-5528.
[26]黄珍玉, 于雁灵, 方彩云, 等. 质谱鉴定磷酸化蛋白研究进展. 质谱学报, 2003, 24(4): 494-500.
[27]Thingholm T E, Jensen O N, Larsen M R. Analytical strategies for phosphoproteomics. Proteomics, 2009, 9(6): 1451-1468.
[28]Peck S C, Nühse T S, Hess D, et al. Directed proteomics identifies a plant-specific protein rapidly phosphorylated in response to bacterial and fungal elicitors. Plant Cell, 2001, 13(6): 1467-1475.
[29]Nühse T S, Bottrill A R, Jones A M, et al. Quantitative phosphoproteomic analysis of plasma membrane proteins reveals regulatory mechanisms of plant innate immune responses. Plant J, 2007, 51(5): 931-940.
[30]Khan M, Takasaki H, Komatsu S. Comprehensive phosphoproteome analysis in rice and identification of phosphoproteins responsive to different hormones/stresses. J Proteome Res, 2005, 4(5): 1592-1599.
[31]Chitteti B R, Peng Z. Proteome and phosphoproteome differential expression under salinity stress in rice (Oryza sativa) roots. J Proteome Res, 2007, 6(5): 1718-1727.
[32]Whiteman S A, Nühse T S, Ashford D A, et al. A proteomic and phosphoproteomic analysis of Oryza sativa plasma membrane and vacuolar membrane. Plant J, 2008, 56(1): 146-156.
[33]Nakagami H, Sugiyama N, Mochida K, et al. Large-scale comparative phosphoproteomics identifies conserved phosphorylation sites in plants. Plant Physiol, 153(3): 1161-1174.
Options
Outlines

/

Tel: 0571-63370278 E-mail: cjrs@263.net
Supported by Beijing Magtech Co., Ltd.