Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2010 , Vol. 24 >Issue 6: 567 - 574

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.06.002

研究报告

Molecular Analysis of Rice CIPKs Involved in Biotic and Abiotic Stress Responses

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  • 1State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China; *Corresponding author, E-mail: hszhang@njau.edu.cn

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2010-11-10

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Abstract

Plant calcineurin Blike (CBL) proteins have been proposed as important Ca2+ sensors and specifically interact with CBLinteracting protein kinases (CIPKs) in plantspecific calcium signaling. We identified and isolated 15 CIPK genes in japonica rice variety Nipponbare based on the predicted sequences of rice CIPK gene family. Gene structure analysis showed that these 15 genes were divided into intronless and intronrich groups, in which OsCIPK3 and OsCIPK24 exhibit alternative splicing. The phylogenetic analysis indicated that rice CIPKs share an ancestor with Arabidopsis and poplar CIPKs. Analysis of gene expression showed that these OsCIPK genes were differentially induced by biotic stress (bacterial blight, BB) and abiotic stresses, including heavy metal (Hg2+), high salt, cold and ABA. Interestingly, five OsCIPK genes (OsCIPK1, OsCIPK2, OsCIPK10, OsCIPK11 and OsCIPK12) were transcriptionally upregulated after BB infection, among which four OsCIPK genes (OsCIPK2, OsCIPK10, OsCIPK11 and OsCIPK14) were induced by all treatments, indicating that some of OsCIPKs were involved in multiple stress response pathways in plants. The findings suggest that CIPKs play a key role in both biotic and abiotic stress responses.

Key words： rice; CIPK family; gene expression; stress; bacterial blight

Cite this article

CHEN Xi-feng,GU Zhi-min,LIU Feng,MA Bo-jun,ZHANG Hong-sheng . Molecular Analysis of Rice CIPKs Involved in Biotic and Abiotic Stress Responses[J]. Chinese Journal OF Rice Science, 2010 , 24(6) : 567 -574 . DOI: 10.3969/j.issn.1001-7216.2010.06.002

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