Effects of Gibberellic Acid 3 and Abscisic Acid on Growth, Physiological Characteristics and Gene Expression of GA20ox2 and GA3ox2 in Different Rice Cultivars

YANG  Yan-hua,ZHANG  Ya-dong,ZHU  Zhen,ZHAO  Ling,CHEN  Tao,ZHAO  Qing-yong,WANG  Cai-lin*

doi:10.3969/j.issn.1001-7216.2010.04.017

2010 , Vol. 24 >Issue 4: 433 - 437

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.04.017

研究简报

Effects of Gibberellic Acid 3 and Abscisic Acid on Growth, Physiological Characteristics and Gene Expression of GA20ox2 and GA3ox2 in Different Rice Cultivars

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Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R & D Center/Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014, China; *Corresponding author， E-mail: clwang@jaas.ac.cn

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-07-10

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Abstract

Effects of exogenous gibberellic acid 3 (GA3) and abscisic acid (ABA) on the growth, physiological characteristics and gene expression of GA20ox2 and GA3ox2 were studied at fourleaf stage with five rice cultivars. Results showed that 1 μmol/L GA3 could promote seedling height, restrain root elongation, and increase the contents of chlorophyll and soluble protein, and permeability of cell membrane. However, 10 mg/L ABA could inhibit the seed germination heavily and also retard seedling height and decrease the contents of chlorophyll and soluble protein, and permeability of cell membrane. RTPCR detection showed that the expression of GA20ox2 was obviously upregulated under the GA3 treatment, except for Nipponbare. On the contrary, ABA restrained the expression of GA20ox2. In the three rice cultivars, Nanjing 44, Kanto 194 and Nipponbare, GA3 induced the expression of GA3ox2 and ABA inhibited the expression of GA3ox2.

Key words： rice; gibberellic acid; abscisic acid; dwarf gene; gene expression; growth; physiological traits

Cite this article

YANG Yan-hua,ZHANG Ya-dong,ZHU Zhen,ZHAO Ling,CHEN Tao,ZHAO Qing-yong,WANG Cai-lin* . Effects of Gibberellic Acid 3 and Abscisic Acid on Growth, Physiological Characteristics and Gene Expression of GA20ox2 and GA3ox2 in Different Rice Cultivars [J]. Chinese Journal OF Rice Science, 2010 , 24(4) : 433 -437 . DOI: 10.3969/j.issn.1001-7216.2010.04.017

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