研究简报

Genetic Analysis and Molecular Mapping of a PhotoperiodThermoSensitive Genic Male Sterile Gene (PTGMS2-1) in Rice Line Guangzhan 63S

Expand
  • 1Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Function Genomics, Ministry of Education, Yangzhou University, Yangzhou 225009, China; 2Lixiahe Region Agricultural Research Institute of Jiangsu, Yangzhou 225007,China; *Corresponding author, E-mail: ricegb@yzu.edu.cn

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2010-07-10

Abstract

Guangzhan 63S, a photoperiodthermosensitive genic male sterile (PTGMS) line, was one of the most widely used PTGMS lines in twoline indica hybrid rice breeding system in China. Genetic analysis indicated that the sterility of Guangzhan 63S was controlled by a single recessive gene using the F2 population derived from a cross between the Guangzhan 63S and Han 1587. Molecular mapping was performed on the F2 mapping population using SSR and InDel markers and bulkedsegregant analysis(BSA). The PTGMS21 was restricted to a 390kb region on chromosome 2 between the markers S24 and S227, at genetic distances of 0.5 cM and 1.1 cM, respectively, and cosegregated with S224.

Cite this article

WANG Bao-he,XU Jian-jun,WU Yin-hui,ZHU Jin-yan,LI Sheng-qiang,ZHOU Yong,CHENG Xiao-tao,LIANG Guo-hua . Genetic Analysis and Molecular Mapping of a PhotoperiodThermoSensitive Genic Male Sterile Gene (PTGMS2-1) in Rice Line Guangzhan 63S[J]. Chinese Journal OF Rice Science, 2010 , 24(4) : 429 -432 . DOI: 10.3969/j.issn.1001-7216.2010.04.016

References

[1]袁隆平. 两系法杂交水稻研究的进展. 中国农业科学, 1990, 23(3): 1-6.
[2]杨振玉, 张国良, 张从合, 等. 中籼型优质光温敏核不育系广占63S的选育. 杂交水稻, 2002, 17(4): 4-6.
[3]Liu N, Shan Y, Wang F P, et al. Identification of an 85-kb DNA fragment containing pms1, a locus for photoperiod-sensitive genic male sterility in rice. Mol Gen Genom, 2001, 266: 271-275.
[4]Lu Q, Li X H, Guo D, et al. Localization of pms3, a gene for photoperiod-sensitive genic male sterility, to a 28.4-kb DNA fragment. Mol Gen Genom, 2005, 273: 507-511.
[5]Wang B, Xu W W, Wang J Z, et al. Tagging and mapping the thermosensitive genic male-sterile gene in rice (Oryza sativa) with molecular markers. Theor Appl Genet, 1995, 91: 1111-1114.
[6]Yamaguchi Y, Ikeda R, Hirasawa H. Linkage analysis of thermosensitive genic male sterility gene tms-2 in rice (Oryza sativa L.). Breeding Sci, 1997, 47(4): 371-373.
[7]Subudhi P K, Borkakati R K, Virmani S S, et al. Molecular mapping of a thermosensitive genetic male-sterility gene in rice using bulked segregant analysis. Genome, 1997, 40: 188-194.
[8]Dong N V, Subudhi P K, Luong P N, et al. Molecular mapping of a rice gene conditioning thermosensitive genic male sterility using AFLP, RFLP and SSR techniques. Theor Appl Genet, 2000, 100: 727-734.
[9]Yang Q K, Liang C Y, Zhuang W, et al. Characterization and identication of the candidate gene of rice thermo-sensitive genic male sterile gene tms5 by mapping. Planta, 2007, 225: 321-330.
[10]Lee D S, Chen L J, Suh H S. Genetic characterization and fine mapping of a novel thermo-sensitive genic male-sterile gene tms6 in rice (Oryza sativa L.). Theor Appl Genet, 2005, 111: 1271-1277.
[11]Jia J H, Zhang D S, Li C Y, et al. Molecular mapping of the reverse thermo-sensitive genic male-sterile gene (rtms1) in rice. Theor Appl Genet, 2001, 103: 607-612.
[12]Koh H J, Son Y H, Heu M H, et al. Molecular mapping of a new genic male-sterility gene causing chalky endosperm in rice (Oryza sativa L.). Euphytica, 1999, 106: 57-62.
[13]Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism(SSLP) in rice(Oryza sativa L.). Mol Gen Genet, 1996, 252: 597-607.
[14]李任华, 王象坤, 罗孝和, 等. 光敏核不育系轮回422S的选育. 杂交水稻, 1995(6): 7-9.
[15]李成荃, 王守海, 许克农, 等. 粳型水稻光敏核不育系7001S的选育及利用研究. 安徽农业, 1996(7): 11.
[16]石明松. 晚粳自然两用系选育及应用初报. 湖北农业科学, 1981(7): 1-3.
[17]Mei M H, Chen L, Zhang Z H. pms3 is the locus causing the original photoperiods ensitive male sterility mutation of “Nongken 58S”. Sci China: Ser C, 1999, 42(3): 316-322.
[18]赵明富, 蔡春苗, 车容会, 等. 水稻光温敏雄性核不育系45S不育基因的分子定位. 分子植物育种, 2008, 6(6): 1045-1049.
[19]李新奇, 袁隆平, 邓华凤, 等. 水稻光敏与温敏核不育基因之间互作效应与利用研究. 科技导报, 2009, 27(3): 74-79.
Outlines

/

Tel: 0571-63370278 E-mail: cjrs@263.net
Supported by Beijing Magtech Co., Ltd.