<FONT face="Verdana">Resistance of Antimicrobial Peptide  Genes Transgenic Rice to Bacterial Blight</FONT>

WANG Wei,WU Chao,LIU Mei,LIU Xu-ri,HU Guo-cheng, SI Hua-min, SUN Zong-xiu, LIU Wen-zhen,FU  Ya-ping

doi:10.3969/j.issn.1001-7216.2010.04.001

Chinese Journal OF Rice Science >

2010 , Vol. 24 >Issue 4: 335 - 340

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.04.001

研究报告

Resistance of Antimicrobial Peptide Genes Transgenic Rice to Bacterial Blight

Expand

¹State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; ² R & D Center of Marine Biotechnology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; ^#These authors contributed equally to this paper; *Corresponding author, Email: lwzzju@163.com; cnrrifuyp@hotmail.com

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-07-10

Fold

Abstract

Antimicrobial peptides (AMP) is a polypeptide with antimicrobial activity. The antimicrobial peptide genes np3 and np5 from Chinese shrimp were integrated into Oryza sativa L. subsp. japonica. cv. Aichi ashahi by Agrobacterium mediated transformation system. PCR analysis showed that the positive ratios of np3 and np5 were 36% and 45% in T0 generation，respectively. RTPCR analysis showed that the antimicrobial peptide genes were expressed in T1 generation and there was no obvious difference in agronomic traits between transgenic plants and nontransgenic plants. Four np3 and np5 transgenic lines in T1 generation were inoculated with the strain CR4 of Xanthomonas oryzae pv. oryzae(Xoo), and the results indicated that all the transgenic lines had a significantly enhanced resistance to bacterial blight caused by the Xoo strain CR4. The np5 transgenic lines also conferred higher resistance to bacterial blight caused by the Xoo strains JS972, ZHE173 and OS225. It is suggested that transgenic lines with np5 gene might possess a broad spectrum resistance to rice bacterial blight.

Key words： rice; antimicrobial peptides; bacterial blight; resistance

Cite this article

WANG Wei,WU Chao,LIU Mei,LIU Xu-ri,HU Guo-cheng, SI Hua-min, SUN Zong-xiu, LIU Wen-zhen,FU Ya-ping . Resistance of Antimicrobial Peptide Genes Transgenic Rice to Bacterial Blight[J]. Chinese Journal OF Rice Science, 2010 , 24(4) : 335 -340 . DOI: 10.3969/j.issn.1001-7216.2010.04.001

References

[1]Mew T M. Current status and future prospects of research on bacterial blight of rice. Ann Rev Phytopathol, 1987, 25: 359-382.

[2]Nino-Liu D O, Darnielle L, Bogdanove A J. A simple method of mass inoculation of rice effective for both pathovars of Xanthomonas oryzae, and the construction of comparable sets of host cDNA libraries spanning early stages of bacterial leaf blight and bacterial leaf streak. J Phytopathol, 2005, 153: 500-504.

[3]Xu S J, Cao Y L, Li X H, et al. Expressional and biochemical characterization of rice disease resistance gene Xa3/Xa26 family. J Integr Plant Biol, 2007, 49: 852-862.

[4]Babu R M, Sajeena A, Samundeeswari A V, et al. Induction of bacterial blight (Xanthomonas oryzae pv. oryzae) resistance in rice by treatment with acibenzolar-S-methyl. Ann Appl Biol， 2003, 143: 333-340.

[5]章琦. 水稻白叶枯病抗性的遗传及改良.北京, 科学出版社, 2007: 130-177.

[6]章琦. 中国杂交水稻白叶枯病抗性的遗传改良.中国水稻科学, 2009, 23(2): 111-119.

[7]Shah J M, Raghupathy V, Veluthambi K. Enhanced sheath blight resistance in transgenic rice expressing an endochitinase gene from Trichoderma virens. Biotechnol Lett, 2009, 31: 239-244.

[8]Takakura Y, Che F S, Ishida Y, et al. Expression of a bacterial flagellin gene triggers plant immune responses and confers disease resistance in transgenic rice plants. Mol Plant Pathol, 2008, 9: 525-529.

[9]Coca M, Penas G, Gomez J, et al. Enhanced resistance to the rice blast fungus Magnaporthe grisea conferred by expression of a cecropin A gene in transgenic rice. Planta, 2006, 223: 392-406.

[10]Jenssen H, Hamill P, Hancock R E. Peptide antimicrobial agents. Clin Microbiol Rev, 2006, 19: 491-511.

[11]Gennaro R, Zanetti M, Benincasa M, et al. Pro-rich antimicrobial peptides from animals: structure,biological functions and mechanism of action. Curr Pharm Des, 2002, 8: 763-778.

[12] 黄大年, 朱冰, 杨炜, 等.抗菌肽B基因导入水稻及转基因植株的鉴定. 中国科学: C辑, 1997, 27(1): 55-62.

[13]Patkar R N, Chattoo B B. Transgenic indica rice expressing ns-LTP-like protein shows enhanced resistance to both fungal and bacterial pathogens. Mol Breeding, 2006, 17: 159-171.

[14]Cuthbertson B J, Bullesbach E E, Gross P S. Discovery of synthetic penaeidin activity against antibiotic-resistant fungi. Chem Biol Drug Des, 2006, 68: 120-127.

[15]Destoumieux D, Munoz M, Bulet P, et al. Penaeidins, a family of antimicrobial peptides from penaeid shrimp (Crustacea, Decapoda). Cell Mol Life Sci, 2000, 57: 1260-1271.

[16]Kang C J, Wang J X. Molecular cloning and expression analysis of Ch-penaeidin, an antimicrobial peptide from Chinese shrimp, Fenneropenaeus chinensis. Fish Shellfish Immunol, 2004, 16: 513-525.

[17]Kang C J, Xue J F. Characterization and expression of a new subfamily member of penaeidin antimicrobial peptides (penaeidin 5) from Fenneropenaeus chinensis. Mol Immunol, 2007, 44: 1535-1543.

[18]Hiei Y, Ohta S, Komari T, et al. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J, 1994, 6: 271-282.

[19]卢扬江, 郑康乐. 提取水稻DNA的一种简易方法.中国水稻科学, 1992, 6(1): 47-48.

[20]华志华，汪晓玲，薛锐, 等. Cecropin B转基因水稻及其后代抗白叶枯病研究初报. 中国水稻科学, 1999, 13(2): 114-116.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References