<FONT face="Verdana">Development of STS Marker KC1 for Brown Planthopper Resistance Gene <EM>Bph18</EM>(t) and Marker Assisted  Selection Efficiency</FONT>

LIANG Yun-tao,#,WANG  Chun-lian,#, LAI Feng-xiang, LIU Pi-qing,WANG Jian,FU Qiang,ZHAO Kai-jun

doi:10.3969/j.issn.1001-7216.2010.03.006

Chinese Journal OF Rice Science >

2010 , Vol. 24 >Issue 3: 244 - 250

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.03.006

研究报告

Development of STS Marker KC1 for Brown Planthopper Resistance Gene Bph18(t) and Marker Assisted Selection Efficiency

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¹Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture/The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081， China； ²Agricultural College, Guangxi University, Nanning 530005, China； ³China National Rice Research Institute, Hangzhou 310006, China；⁴ Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China； ^# These authors contributed equally to this paper； *Corresponding author, E-mail： zhaokj@mail.caas.net.cn

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-05-10

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Abstract

To facilitate application of the elite brown planthopper(BPH) resistance gene Bph18(t) to rice breeding program by molecular assisted selection(MAS), a STS marker, KC1, for Bph18(t) gene was developed. To test the MAS efficiency of KC1 for Bph18(t), the F2 plants derived from the cross between Yangdao 6(i.e.9311)\[without Bph18(t), susceptible to BPH\] and C4064 \[harboring Bph18(t)\] were subjected to both bioassay of BPH resistance in the field and molecular identification with the STS marker KC1 in the laboratory. Among the F2 plants, 292 plants were resistant to BPH while the other 84 plants were susceptible, indicating a single gene \[Bph18(t)\] controlling the BPH resistance in the F2 population. The results of molecular identification showed that the MAS efficiency of KC1 for Bph18(t) was 82.6%, indicating that the STS marker KC1 could be useful to trail Bph18(t) gene in rice breeding program.

Key words： resistance gene; molecular marker; rice brown planthopper; marker assisted selection

Cite this article

LIANG Yun-tao,#,WANG Chun-lian,#, LAI Feng-xiang, LIU Pi-qing,WANG Jian,FU Qiang,ZHAO Kai-jun . Development of STS Marker KC1 for Brown Planthopper Resistance Gene Bph18(t) and Marker Assisted Selection Efficiency[J]. Chinese Journal OF Rice Science, 2010 , 24(3) : 244 -250 . DOI: 10.3969/j.issn.1001-7216.2010.03.006

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