Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2010 , Vol. 24 >Issue 2: 125 - 130

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.02.04

研究报告

Cloning and Expression Analysis of a MitogenActivated Protein Kinase Gene OsMPK14 from Rice

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  • College of Life Sciences, Henan Normal University, Xinxiang 453007, China; *Corresponding author, Email: liangwh@henannu.edu.cn

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2010-03-10

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Abstract

Mitogen activatedprotein kinases (MAPKs) are important components in signal transduction pathways responding to various biotic and abiotic stresses. The cDNA sequence of the MAPK gene OsMPK14 (GenBank Accession No. GQ265780) from rice (Oryza sativa L.) was cloned by RTPCR. The fulllength cDNA of OsMPK14 is 1660 bp in size，containing an open reading frame of 1629 bp，which encodes a 542aminoacid polypeptide and has a typical protein kinase domain and a phosphorylation activation motif TDY. The sequence alignment and analysis revealed that OsMPK14 is located on chromosome 5, and composed of 9 exons and 8 introns in the coding region. Semiquantitative RTPCR was performed to examine its expression patterns in rice shoots and roots under darkness, drought, high salinity, low temperature and abscisic acid treatments. The OsMPK14 mRNA was induced by abscisic acid, low temperature and high salinity, but weakly inhibited by drought. Light could upregulate its expression in roots, but downregulate in shoots. The results indicate that OsMPK14 could be implicated in diverse rice stimuliresponsive signaling cascades, and its expression might be regulated by multiple factors.

Key words： rice; mitogenactivated protein kinase gene; gene clone; stress; expression analysis

Cite this article

LIANG Weihong*, BI Jiajia,PENG Weifeng,ZHANG Fan,SHI Honghao,LI Li . Cloning and Expression Analysis of a MitogenActivated Protein Kinase Gene OsMPK14 from Rice[J]. Chinese Journal OF Rice Science, 2010 , 24(2) : 125 -130 . DOI: 10.3969/j.issn.1001-7216.2010.02.04

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