<FONT face="Verdana">Rice Blast Resistance of Transgenic Rice Plants with Pi-d2 Gene </FONT>

CHEN De-xi,CHEN Xue-wei,LEI Cai-lin,MA Bing-tian,WANG Yu-ping,LI Shi-gui

doi:10.3969/j.issn.1001-7216.2010.01.06

Chinese Journal OF Rice Science >

2010 , Vol. 24 >Issue 1: 31 - 35

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.01.06

研究报告

Rice Blast Resistance of Transgenic Rice Plants with Pi-d2 Gene

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1Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China; 2Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China； 3Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; *Corresponding author, E-mail: lishigui_sc@263.net)

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-01-10

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Abstract

Transgenic rice lines harboring Pid2 gene under the control of different promoters from three expression vector pCB6.3kb, pCB53kb and pZH01272kb were tested for resistance to Magnaporthe grisea. The results showed that 9 stably transgenic lines had different resistances to 39 strains of M. grisea from Sichuan Province, China, and the highest diseaseresistance frequency was 917%. Four early homozygous transgenic lines of Pid2 gene exhibited resistance to more than 8148% of 58 strains from Chinese Academy of Agricultural Sciences, which indicates that the transgenic rice lines possessed widespectrum resistance against various rice blast races. The results of transgenic embryonic callus selected by crude toxin showed that the induction rate of embryonic callus reduced by increased concentration of toxin in medium. When the toxin concentration was 40% in medium, the induction rate of embryonic callus of transgenic plant was 4933%, while that of the receptor control was 5%. The disease incidence of neck blast were 0% to 50% in the transgenic lines under induction of rice blast disease in fields, showing the higher resistance to rice blast than that of receptor control.

Key words： transgenic rice; resistance gene; rice blast; resistance spectrum; crude toxin

Cite this article

CHEN De-xi,CHEN Xue-wei,LEI Cai-lin,MA Bing-tian,WANG Yu-ping,LI Shi-gui . Rice Blast Resistance of Transgenic Rice Plants with Pi-d2 Gene [J]. Chinese Journal OF Rice Science, 2010 , 24(1) : 31 -35 . DOI: 10.3969/j.issn.1001-7216.2010.01.06

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